Reference Report

Reference
Citation	Miron, M., Verdu, J., Lachance, P.E.D., Birnbaum, M.J., Lasko, P.F., Sonenberg, N. (2001). The translational inhibitor 4E-BP is an effector of PI(3)K/Akt signalling and cell growth in Drosophila. Nat. Cell Biol. 3(6): 596--601. (Export to RIS)
FlyBase ID	FBrf0137059
Publication Type	Research paper
External Crossreferences
PubMed ID	11389445
PubMed Abstract	The initiation factor 4E for eukaryotic translation (eIF4E) binds the messenger RNA 5'-cap structure and is important in the regulation of protein synthesis. Mammalian eIF4E activity is inhibited when the initiation factor binds to the translational repressors, the 4E-binding proteins (4E-BPS). Here we show that the Drosophila melanogaster 4E-BP (d4E-BP) is a downstream target of the phosphatidylinositol-3-OH kinase (PI(3)K) signal-transduction cascade, which affects the interaction of d4E-BP with eIF4E. Ectopic expression of a highly active d4E-BP mutant in wing-imaginal discs causes a reduction of wing size, brought about by a decrease in cell size and number. A marked reduction in cell size was also observed in post-mitotic cells. Expression of d4E-BP in the eye and wing together with PI(3)K or dAkt1, the serine/threonine kinase downstream of PI(3)K, resulted in suppression of the growth phenotype elicited by these kinases. Our results support a role for d4E-BP as an effector of cell growth.
BIOSIS ID	2001.354572
Zoological Record ID
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Secondary IDs
Language of Publication	English
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ISBN
Parent Publication
Publication Type	Journal
Abbreviation	Nat. Cell Biol.
Title	Nature Cell Biology
Editors
Publication Year	1999-
ISBN/ISSN	1465-7392 1476-4679
Data from Reference
Aberrations (1)
	Df(2L)Pi3K21B-A
Alleles (12)
	Akt1^{Scer\UAS.T:Ivir\HA1} BacA\p35^Scer\UAS.cHa Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} Pi3K92E^{Scer\UAS.T:Hsap\MYC} Scer\GAL4^Act5C.PP Scer\GAL4^Bx-MS1096 Scer\GAL4^dpp.blk1 Scer\GAL4^GMR.PF Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} Thor^LL.Scer\UAS Thor^Scer\UAS.cMa w^+mC
Constructs (11)
	P{Dp110^D954A} P{GAL4-Act5C(FRT.CD2).P} P{GAL4-Act5C(-FRT).P} P{GAL4-dpp.blk1} P{GAL4-ninaE.GMR} P{U2af38.gR10} P{UAS-Akt1.HA} P{UAS-p35.H} P{UAS-Thor.LL} P{UAS-Thor.wt} P{WT-Dp110}
Genes (10)
	Akt1 BacA\p35 eIF-4E Pi3K21B Pi3K92E Scer\GAL4 T:Hsap\MYC T:Ivir\HA1 Thor w
Insertions (3)
	P{GawB}Bx^MS1096 P{UAS-Thor.LL}s P{UAS-Thor.LL}w