Reference Report

Reference
Citation	Lee, M.J., Gergely, F., Jeffers, K., Peak-Chew, S.Y., Raff, J.W. (2001). Msps/XMAP215 interacts with the centrosomal protein D-TACC to regulate microtubule behaviour. Nat. Cell Biol. 3(7): 643--649. (Export to RIS)
FlyBase ID	FBrf0137062
Publication Type	Research paper
PubMed ID	11433296
PubMed Abstract	The XMAP215/ch-TOG/Msps family of microtubule-associated proteins (MAPs) promote microtubule growth in vitro and are concentrated at centrosomes in vivo. We show here that Msps (mini-spindles protein) interacts with the centrosomal protein D-TACC, and that this interaction strongly influences microtubule behaviour in Drosophila embryos. If D-TACC levels are reduced, Msps does not concentrate at the centrosomes efficiently and the centrosomal microtubules appear to be destabilized. If D-TACC levels are increased, both D-TACC and Msps accumulate around the centrosomes/spindle poles, and the centrosomal microtubules appear to be stabilized. We show that the interaction between D-TACC and Msps is evolutionarily conserved. We propose that D-TACC and Msps normally cooperate to stabilize centrosomal microtubules by binding to their minus ends and binding to their plus ends as they grow out from the centrosome.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nat. Cell Biol.
Title	Nature Cell Biology
Publication Year	1999-
ISBN/ISSN	1465-7392 1476-4679
Data from Reference
Alleles (11)
	msps^MJ15 msps^T:Avic\GFP Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} Sjap\GST^{Scer\UAS.P\T.cLa} T:Avic\GFP^m6 tacc¹ tacc^stella592 tacc^{TD.Scer\UAS.P\T.T:Sjap\GST} tacc^Ubi-p63E.PG tacc^{Ubi-p63E.T:Avic\GFP-m6} w^+mC
Constructs (6)
	P{matα4-GAL-VP16} P{msps-GFP} P{UASp-GST.L} P{UASp-tacc.GST-TD} P{Ubi-tacc.GFP} P{Ubi-tacc}
Genes (12)
	Cp190 Map60 msps Scer\GAL4 Sjap\GST T:Avic\GFP T:Hsim\VP16 T:Sjap\GST tacc w γTub23C γTub37C
Insertions (1)
	P{}tacc^stella592