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| Citation | Larochelle, S., Chen, J., Knights, R., Pandur, J., Morcillo, P., Erdjument-Bromage, H., Tempst, P., Suter, B., Fisher, R.P. (2001). T-loop phosphorylation stabilizes the CDK7-cyclin H-MAT1 complex in vivo and regulates its CTD kinase activity. EMBO J. 20(14): 3749--3759. (Export to RIS) | ||
| FlyBase ID | FBrf0137227 | ||
| Publication Type | Research paper | ||
| PubMed ID | 11447116 | ||
| PubMed Abstract | Cyclin-dependent kinase (CDK)7-cyclin H, the CDK-activating kinase (CAK) and TFIIH-associated kinase in metazoans can be activated in vitro through T-loop phosphorylation or binding to the RING finger protein MAT1. Although the two mechanisms can operate independently, we show that in a physiological setting, MAT1 binding and T-loop phosphorylation cooperate to stabilize the CAK complex of Drosophila. CDK7 forms a stable complex with cyclin H and MAT1 in vivo only when phosphorylated on either one of two residues (Ser164 or Thr170) in its T-loop. Mutation of both phosphorylation sites causes temperature-dependent dissociation of CDK7 complexes and lethality. Furthermore, phosphorylation of Thr170 greatly stimulates the activity of the CDK7- cyclin H-MAT1 complex towards the C-terminal domain of RNA polymerase II without significantly affecting activity towards CDK2. Remarkably, the substrate-specific increase in activity caused by T-loop phosphorylation is due entirely to accelerated enzyme turnover. Thus phosphorylation on Thr170 could provide a mechanism to augment CTD phosphorylation by TFIIH-associated CDK7, and thereby regulate transcription. | ||
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| Language of Publication | English | ||
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| Publication Type | Journal | ||
| Abbreviation | EMBO J. | ||
| Title | The EMBO Journal | ||
| Publication Year | 1982- | ||
| ISBN/ISSN | 0261-4189 | ||
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Data from Reference
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| Aberrations (1)
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| Alleles (5)
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| Constructs (4)
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| Genes (5)
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