Reference Report

Reference
Citation	Amikura, R., Kashikawa, M., Nakamura, A., Kobayashi, S. (2001). Presence of mitochondria-type ribosomes outside mitochondria in germ plasm of Drosophila embryos. Proc. Natl. Acad. Sci. U.S.A. 98(16): 9133--9138. (Export to RIS)
FlyBase ID	FBrf0137346
Publication Type	Research paper
PubMed ID	11470924
PubMed Abstract	Mitochondrially encoded large and small ribosomal RNAs (mtlrRNA and mtsrRNA) are transported out of mitochondria to polar granules, the distinctive organelles of germ plasm in Drosophila. Reduction of the extramitochondrial mtlrRNA amount leads to the failure of embryos to form the germ-line progenitors, or pole cells, suggesting that mtlrRNA, along with mtsrRNA, functions on the polar granules to specify the germ line. In this study, we provide several lines of evidence showing that there are mitochondria-type ribosomes on the polar granules during a short period before pole cell formation. Our ultrastructural analysis reveals that these ribosomes include both mitochondrial rRNAs and at least two mitochondrial ribosomal proteins (S12 and L7/L12). Furthermore, these ribosomes are integrated into well developed polysomes on the surface of polar granules. We propose that translation dependent on mitochondria-type ribosomes is an important mechanism underlying germ-line formation.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (5)
	mRpL12^{Scer\UAS.P\T.T:Avic\GFP-EGFP} mRpL12^{Scer\UAS.P\T.T:Ivir\HA1} Scer\GAL4^{nos.UTR.T:Hsim\VP16} T:Avic\GFP^EGFP tko^{Scer\UAS.P\T.T:Avic\GFP-EGFP}
Constructs (4)
	P{GAL4::VP16-nos.UTR} P{UASp-mRpL7-L12.T:Avic\GFP.A} P{UASp-mRpL7-L12.T:Ivir\HA1.A} P{UASp-tko.T:Avic\GFP.A}
Genes (6)
	mRpL12 Scer\GAL4 T:Avic\GFP T:Hsim\VP16 T:Ivir\HA1 tko