Reference Report

Reference
Citation	Ragone, G., Bernardoni, R., Giangrande, A. (2001). A novel mode of asymmetric division identifies the fly neuroglioblast 6-4T. Dev. Biol. 235(1): 74--85. (Export to RIS)
FlyBase ID	FBrf0137378
Publication Type	Research paper
PubMed ID	11412028
PubMed Abstract	Asymmetric cell divisions and segregation of fate determinants are crucial events in the generation of cell diversity. Fly neuroblasts, the precursors that self-reproduce and generate neurons, represent a clear example of asymmetrically dividing cells. Less is known about how neurons and glial cells are generated by multipotent precursors. Flies provide the ideal model system to study this process. Indeed, neuroglioblasts (NGBs) can be specifically identified and have been shown to require the glide/gcm fate determinant to produce glial cells, which otherwise would become neurons. Here, we follow the division of a specific NGB (NGB6-4T), which produces a neuroblast (NB) and a glioblast (GB). We show that, to generate the glioblast, glide/gcm RNA becomes progressively unequally distributed during NGB division and preferentially segregates. Subsequently, a GB-specific factor is required to maintain glide/gcm expression. Both processes are necessary for gliogenesis, showing that the glial vs. neuronal fate choice is a two-step process. This feature, together with glide/gcm subcellular RNA distribution and the behavior of the NGB mitotic apparatus identify a novel type of division generating cell diversity.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (1)
	Df(3R)I9
Alleles (7)
	gcm^N7-4 gcm^Scer\UAS.cBa pros¹⁷ pros^Scer\UAS.cMa Scer\GAL4^eg-Mz360 Scer\GAL4^sca-4512 stau²
Constructs (2)
	P{UAS-gcm.B} P{UAS-pros.17K-2}
Genes (8)
	eg gcm His3 mira pros repo Scer\GAL4 stau
Insertions (2)
	P{GAL4}sca⁴⁵¹² P{GawB}eg^Mz360