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Reference Report
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| Citation | Millburn, G. (2001.4.25). FlyBase error report for CG15137 on Wed Apr 25 09:22:53 2001. (Export to RIS) | ||
| FlyBase ID | FBrf0137430 | ||
| Publication Type | Personal communication to FlyBase | ||
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| PubMed Abstract | |||
| Text of Personal Communication |
From FlyBase-error@XXXXXXXXXXXXXXX Wed Apr 25 17:22:56 2001
Envelope-to: gm119@XXXXXXXXXXXXXXX Delivery-date: Wed, 25 Apr 2001 17:22:56 \+0100 From: FlyBase-error@XXXXXXXXXXXXXXX Date: Wed, 25 Apr 2001 09:22:53 \-0700 (PDT) X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error using \-f To: flybase-updates@XXXXXXXXXXXXXXX Cc: gm119@XXXXXXXXXXXXXXX Subject: FlyBase error report for CG15137 on Wed Apr 25 09:22:53 2001 Content-Length: 786 Error report from Gillian Millburn (gm119@XXXXXXXXXXXXXXX) Gene or accession: CG15137 Release: 1 Gene annotation error Gene CG15137 should be split. Comments: http://cpmcnet.columbia.edu/dept/neurobeh/axel/gr.html (being curated as Scott and Axel, 2001.4.25, personal communication to FlyBase) describes 2 gustatory receptor (Gr) genes: Gr36B.2 and Gr36B.3, both of which overlap somewhat with CG15137, suggesting that CG15137 may need splitting into 2 genes, or Gr36B.2 and Gr36B.3 may need merging (the alignment of Gr36B.2 with CG15137 is not that great, so I'm not sure what needs to happen). alignments of the 2 Gr genes to CG15137 follow in e-mail to flybase-updates Gillian Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) Accessed from: query.pl, /www/hedgehog_8001/htdocs From gm119@XXXXXXXXXXXXXXX Wed Apr 25 17:24:20 2001 Envelope-to: gm119@XXXXXXXXXXXXXXX Delivery-date: Wed, 25 Apr 2001 17:24:20 \+0100 To: flybase-updates@XXXXXXXXXXXXXXX Subject: CG15137 alignments (for FlyBase error report) X-Sun-Charset: US-ASCII From: Gillian Millburn (Genetics) <gm119@XXXXXXXXXXXXXXX> Date: Wed, 25 Apr 2001 17:24:14 \+0100 Content-Length: 4658 here are alignments BLASTP of GR against predicted proteins: Query= Gr36B2 , 390 bases, A460EF58 checksum. (390 letters) Database: aa_gadfly.dros 14,080 sequences; 6,850,524 total letters. Searching....10....20....30....40....50....60....70....80....90....100% done Smallest Sum High Probability Sequences producing High-scoring Segment Pairs: Score P(N) N CG15137|FBan0015137|CT35034|FBan0015137 last_updated:000321 440 2.0e-50 2 >CG15137|FBan0015137|CT35034|FBan0015137 last_updated:000321 Length = 361 Score = 440 (154.9 bits), Expect = 2.0e-50, Sum P(2) = 2.0e-50 Identities = 82/201 (40%), Positives = 132/201 (65%) Query: 90 LITVLNRWLQRGQMMQLVKDVIRLYMINPQLKSMIRWGILLKAFISFAIELLQVTLSVDA 149 L T++ RW QR \+MM L V+R+Y+ PQ++ M RWGIL K \+ LQ+ \+ \+ A Sbjct: 131 LFTLILRWYQRCKMMDLASKVVRMYVARPQVRRMSRWGILTKFIFGSITDGLQMAMVLSA 190 Query: 150 LDRQGTAEMMGLLVKLCVSFIMNLAISQHFLVILLIRAQYRIMNAKLRMVIEESRRLSFL 209 \+ \+ \+GL \++ \+ I+N+A+ Q \+++L \+R Q++++N \+LR VI+E++ L Sbjct: 191 MGSVDSQFYLGLGLQYWMFVILNMAMMQQHMIMLFVRTQFQLINTELRQVIDEAKDLLLS 250 Query: 210 QLRNGAFMTRCCYLSDQLEDIGEVQSQLQSMVGQLDEVFGMQGLMAYSEYYLSIVGTSYM 269 G FMT+CC L+DQ+E+I \+QSQLQ+++ Q++EVFG+QG M Y YYLS VGT Y+ Sbjct: 251 PRHQGVFMTKCCSLADQIENIARIQSQLQTIMNQMEEVFGIQGAMTYGGYYLSSVGTCYL 310 Query: 270 SYSIYKYGPHNLKLSAKTSII 290 \+YSI K+G NL \++ T I+ Sbjct: 311 AYSILKHGYENLSMTLSTVIL 331 Score = 281 (98.9 bits), Expect = 4.7e-26, P = 4.7e-26 Identities = 60/81 (74%), Positives = 66/81 (81%) Query: 260 YLSIVGTSYMSYSIYKYGPHNLKLSAKTSIIVCILITLFYLDALVNCNNMLRVLDHHKDF 319 \+L \+ \++M+ Y L+ AKTSIIVCILITLFYLDALVNCNNMLRVLDHHKDF Sbjct: 17 FLQLRNGAFMTRCCYL--SDQLEDIAKTSIIVCILITLFYLDALVNCNNMLRVLDHHKDF 74 Query: 320 LGLLEERTVFASSLDIRLEES 340 LGLLEERTVFASSLDIRLEES Sbjct: 75 LGLLEERTVFASSLDIRLEES 95 Score = 200 (70.4 bits), Expect = 5.7e-15, P = 5.7e-15 Identities = 42/76 (55%), Positives = 53/76 (69%) Query: 192 MNAKLRMVIEESRRLSFLQLRNGAFMTRCCYLSDQLEDIGEVQSQLQSMVG--QLDEVFG 249 MNAKLRMVIEESRRLSFLQLRNGAFMTRCCYLSDQLEDI \+ \+ \++ LD \+ Sbjct: 1 MNAKLRMVIEESRRLSFLQLRNGAFMTRCCYLSDQLEDIAKTSIIVCILITLFYLDALVN 60 Query: 250 MQGLMAYSEYYLSIVG 265 \++ \+++ \+G Sbjct: 61 CNNMLRVLDHHKDFLG 76 Score = 88 (31.0 bits), Expect = 2.0e-50, Sum P(2) = 2.0e-50 Identities = 19/34 (55%), Positives = 23/34 (67%) Query: 1 MVDWVVLLLKAVHIYCYLIGLSNFEFDCRTGRVF 34 \++D LL AV+ Y IGLSNFEFD TGR+F Sbjct: 99 IMDLESFLLGAVYYYGLFIGLSNFEFDWNTGRLF 132 Query= Gr36B3 , 344 bases, CF996511 checksum. (344 letters) Database: aa_gadfly.dros 14,080 sequences; 6,850,524 total letters. Searching....10....20....30....40....50....60....70....80....90....100% done Smallest Sum High Probability Sequences producing High-scoring Segment Pairs: Score P(N) N CG15137|FBan0015137|CT35034|FBan0015137 last_updated:000321 1053 7.3e-108 1 >CG15137|FBan0015137|CT35034|FBan0015137 last_updated:000321 Length = 361 Score = 1053 (370.7 bits), Expect = 7.3e-108, P = 7.3e-108 Identities = 212/228 (92%), Positives = 215/228 (94%) Query: 89 TG-LFTLILRWYQRCKMMDLASKVVRMYVARPQVRRMSRWGILTKFIFGSITDGLQMAMV 147 TG LFTLILRWYQRCKMMDLASKVVRMYVARPQVRRMSRWGILTKFIFGSITDGLQMAMV Sbjct: 128 TGRLFTLILRWYQRCKMMDLASKVVRMYVARPQVRRMSRWGILTKFIFGSITDGLQMAMV 187 Query: 148 LSAMGSRVDSQFYLGLGLQYWMFVILNMAMMQQHMIMLFVRTQFQLINTELRQVIDEAKD 207 LSAMGS VDSQFYLGLGLQYWMFVILNMAMMQQHMIMLFVRTQFQLINTELRQVIDEAKD Sbjct: 188 LSAMGS-VDSQFYLGLGLQYWMFVILNMAMMQQHMIMLFVRTQFQLINTELRQVIDEAKD 246 Query: 208 LLLSPRHQGVFMTKCCSLADQIENIARIQSQLQTIMNQMEEVFGIQGAMTYGGYYLSSVG 267 LLLSPRHQGVFMTKCCSLADQIENIARIQSQLQTIMNQMEEVFGIQGAMTYGGYYLSSVG Sbjct: 247 LLLSPRHQGVFMTKCCSLADQIENIARIQSQLQTIMNQMEEVFGIQGAMTYGGYYLSSVG 306 Query: 268 TCYLAYSILKHGYENLSMTLSTVILAYSW-----CFFYYLDGMLNLSVM 311 TCYLAYSILKHGYENLSMTLSTVILAYSW C+ Y G \+L+ M Sbjct: 307 TCYLAYSILKHGYENLSMTLSTVILAYSWMTTGKCYKYSGSGQYSLAWM 355 |
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| Language of Publication | English | ||
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