Reference Report

Reference
Citation	Calvi, B.R., Spradling, A.C. (2001). The nuclear location and chromatin organization of active chorion amplification origins. Chromosoma 110(3): 159--172. (Export to RIS)
FlyBase ID	FBrf0138258
Publication Type	Research paper
PubMed ID	11513291
PubMed Abstract	It remains unclear how certain regions on metazoan chromosomes are selected to initiate DNA replication. In recent years a number of origins of DNA replication have been mapped, but there is still no DNA consensus for predicting where replication will initiate. Evidence suggests that the higher order structure of the nucleus and chromosome influences origin activity. Chromosomal DNA replication is proposed to occur in special compartments in the nucleus called replication foci. Foci in different regions of the nucleus initiate replication at different times of S-phase, suggesting nuclear position may contribute to where and when replication begins. Here we test the contribution of nuclear compartments for well-defined origins, those involved in amplification of the chorion (eggshell) genes during Drosophila oogenesis. The results of three-dimensional confocal microscopy indicate that chorion DNA replication origins are highly active in diverse positions within the nucleus. We also find that chorion replication origins inserted at ectopic chromosomal sites can amplify highly in diverse nuclear locations distinct from the endogenous loci, including when they are buffered against genomic position effects. We used fluorescence in situ hybridization to analyze chromosome structure during amplification. Contrary to the replication factory model, we find no evidence for spooling of DNA toward a replication center. We discuss the implications of these results for understanding the role of higher order structure in amplification and chromosome duplication.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Chromosoma
Title	Chromosoma
Publication Year	1939-
ISBN/ISSN	0009-5915
Data from Reference
Alleles (1)
	gypsy\su(Hw)BR⁴³⁰
Constructs (1)
	P{3.8S-Y}
Genes (5)
	gypsy\su(Hw)BR Ori66Dβ ry su(Hw) y
Insertions (3)
	P{3.8S-Y}15 P{3.8S-Y}34 P{S6.9B}5