Reference Report

Reference
Citation	Lee, L.A., Elfring, L.K., Bosco, G., Orr-Weaver, T.L. (2001). A genetic screen for suppressors and enhancers of the Drosophila pan gu cell cycle kinase identifies cyclin B as a target. Genetics 158(4): 1545--1556. (Export to RIS)
FlyBase ID	FBrf0138402
Publication Type	Research paper
PubMed ID	11514446
PubMed Abstract	The early cell cycles of Drosophila embryogenesis involve rapid oscillations between S phase and mitosis. These unique S-M cycles are driven by maternal stockpiles of components necessary for DNA replication and mitosis. Three genes, pan gu (png), plutonium (plu), and giant nuclei (gnu) are required to control the cell cycle specifically at the onset of Drosophila development by inhibiting DNA replication and promoting mitosis. PNG is a protein kinase that is in a complex with PLU. We employed a sensitized png mutant phenotype to screen for genes that when reduced in dosage would dominantly suppress or enhance png. We screened deficiencies covering over 50% of the autosomes and identified both enhancers and suppressors. Mutations in eIF-5A and PP1 87B dominantly suppress png. Cyclin B was shown to be a key PNG target. Mutations in cyclin B dominantly enhance png, whereas png is suppressed by cyclin B overexpression. Suppression occurs via restoration of Cyclin B protein levels that are decreased in png mutants. The plu and gnu phenotypes are also suppressed by cyclin B overexpression. These studies demonstrate that a crucial function of PNG in controlling the cell cycle is to permit the accumulation of adequate levels of Cyclin B protein.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (103)
	Df(1)bi-D3 Df(2L)30A-C Df(2L)al Df(2L)ast2 Df(2L)b87e25 Df(2L)C144 Df(2L)cl-h3 Df(2L)dp-79b Df(2L)E55 Df(2L)E110 Df(2L)esc10 Df(2L)H20 Df(2L)JS32 Df(2L)net-PMF Df(2L)osp29 Df(2L)pr76 Df(2L)Prl Df(2L)r10 Df(2L)spd Df(2L)TW50 Df(2L)TW84 Df(2L)TW137 Df(2R)44CE Df(2R)59AB Df(2R)59AD Df(2R)173 Df(2R)AA21 Df(2R)B5 Df(2R)cn9 Df(2R)cn88b Df(2R)CX1 Df(2R)en30 Df(2R)en-B Df(2R)H3C1 Df(2R)H3E1 Df(2R)Jp1 Df(2R)Jp8 Df(2R)l13 Df(2R)M41A4 Df(2R)M60E Df(2R)nap1 Df(2R)nap9 Df(2R)P34 Df(2R)PC4 Df(2R)Pcl7B Df(2R)Pcl11B Df(2R)pk78s Df(2R)Pu-D17 Df(2R)Px2 Df(2R)stan2 Df(2R)trix Df(2R)vg56 Df(2R)vg135 Df(2R)vg-B Df(2R)vg-C Df(2R)X1 Df(2R)X58-7 Df(2R)X58-12 Df(3L)29A6 Df(3L)66C-G28 Df(3L)81k19 Df(3L)Ar14-8 Df(3L)BK10 Df(3L)brm11 Df(3L)Cat Df(3L)emc-E12 Df(3L)fz-GF3b Df(3L)fz-M21 Df(3L)GN24 Df(3L)GN50 Df(3L)h-i22 Df(3L)HR119 Df(3L)Ly Df(3L)R-G5 Df(3L)R-G7 Df(3L)ri-79c Df(3L)st-f13 Df(3L)vin7 Df(3L)VW3 Df(3L)W4 Df(3L)W10 Df(3R)2-2 Df(3R)3450 Df(3R)awd-KRB Df(3R)C4 Df(3R)Cha7 Df(3R)crb87-4 Df(3R)crb87-5 Df(3R)Dl-BX12 Df(3R)e-BS2 Df(3R)e-N19 Df(3R)e-R1 Df(3R)L127 Df(3R)P14 Df(3R)p712 Df(3R)p-XT103 Df(3R)ry615 Df(3R)Scr Df(3R)Tpl10 Dp(3;1)2-2 In(2LR)Px⁴ In(2R)bw^VDe2LCy^R In(3R)Ubx^7LLats^R
Alleles (44)
	Aats-asp^k04508 cdc2^B47 Cul-3^unspecified CycA⁵ CycA^hs.PK CycB3² CycB3^+t9 CycB² CycB^+t10 CycB^{D46.Scer\UAS.P\T} CycB^hs.PK CycE⁰⁵²⁰⁶ dap⁰⁴⁴⁵⁴ Dp^unspecified dup^unspecified E2f^unspecified eff^unspecified eIF-3p40^k09003 eIF-4a^k01501 eIF-4E⁰⁷²³⁸ eIF-5A⁰¹²⁹⁶ eIF-5A¹⁰⁵³⁰ esg^unspecified fzy^unspecified gnu³⁰⁵ grp^unspecified ida^B4 l(2)10481¹⁰⁴⁸¹ l(2)s4989^s4989 lwr^unspecified mr^unspecified mts^k09822 mts^s5286 pim^unspecified plu⁶ png¹⁰⁵⁸ png³³¹⁸ png^unspecified Pp1-87B^j6E7 Rca1^unspecified RpL30^k00308 Scer\GAL4^{nos.UTR.T:Hsim\VP16} thr^unspecified w^+mC
Constructs (6)
	P{CycB3⁺.J} P{CycB⁺.J} P{GAL4::VP16-nos.UTR} P{hs-CycA} P{hs-CycB} P{UASp-CycB.D46}
Genes (39)
	Aats-asp cdc2 CG16778 Cul-3 CycA CycB CycB3 CycE CycJ dap Dp dup E2f eff eIF-3p40 eIF-4a eIF-4E eIF-5A esg fzy gnu grp ida l(2)10481 l(2)s4989 lwr mr mts pim plu png Pp1-87B rap Rca1 RpL30 Scer\GAL4 T:Hsim\VP16 thr w
Insertions (2)
	P{lacW}s4989 P{PZ}l(2)10481¹⁰⁴⁸¹