Reference Report

Reference
Citation	Raymond, K., Bergeret, E., Dagher, M.C., Breton, R., Griffin-Shea, R., Fauvarque, M.O. (2001). The Rac GTPase-activating protein RotundRacGAP interferes with Drac1 and Dcdc42 signalling in Drosophila melanogaster. J. Biol. Chem. 276(38): 35909--35916. (Export to RIS)
FlyBase ID	FBrf0138438
Publication Type	Research paper
PubMed ID	11468292
PubMed Abstract	RhoGTPases are negatively regulated by GTPase-activating proteins (GAPs). Here we demonstrate that Drosophila RotundRacGAP is active in vitro on Drac1 and Dcdc42 but not Drho1. Similarly, in yeast, RotundRacGAP interacts specifically with Drac1 and Dcdc42, as well as with their activated V12 forms, showing a particularly strong interaction with Dcdc42V12. In the fly, lowering RotundRacGAP dosage specifically modifies eye defects induced by expressing Drac1 or Dcdc42 but not Drho1, confirming that Drac1 and Dcdc42 are indeed in vivo targets of RotundRacGAP. Furthermore, embryonic-directed expression of either RotundRacGAP, or dominant negative Drac1N17, transgenes induces similar defects in dorsal closure and inhibits Drac1-dependent cytoskeleton assembly at the leading edge. Expression of truncated forms of RotundRacGAP shows that the GAP domain of RotundRacGAP is essential for its function. Unexpectedly, transgenes encoding Drac1N17, Dcdc42N17, or RotundRacGAP do not affect the c-Jun N-terminal kinase-dependent gene expression of decapentaplegic and puckered, indicating that another Drac1-independent signal redundantly activates this pathway. Finally, in a situation where Drac1 is constitutively activated, RotundRacGAP greatly reduces the ectopic expression of decapentaplegic, possibly by negatively regulating Dcdc42.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	J. Biol. Chem.
Title	Journal of Biological Chemistry
Publication Year	1905-
ISBN/ISSN	0021-9258
Data from Reference
Aberrations (1)
	Df(3R)rn20
Alleles (18)
	Cdc42^GMR.PN Cdc42^N17.Scer\UAS Ecol\lacZ^puc-E69 Ecol\lacZ^Scer\UAS.cUa hep¹ hep^CA.Scer\UAS Rac1^GMR.PN Rac1^N17.Scer\UAS Rac1^V12.Scer\UAS RacGAP84C^GAP.Scer\UAS RacGAP84C^Scer\UAS.cRa RacGAP84C^Zn.Scer\UAS Rho1^GMR.PH Scer\GAL4^69B Scer\GAL4^LE Scer\GAL4^pnr-MD237 Scer\GAL4^ptc-559.1 w^+mC
Constructs (11)
	P{GMR-Cdc42.N} P{GMR-Rac1.N} P{GMR-Rho1} P{UAS-Cdc42.N17} P{UAS-hep.CA} P{UAS-lacZ.U} P{UAS-Rac1.N17} P{UAS-Rac1.V12} P{UAS-rnRacGAP.GAP} P{UAS-rnRacGAP.R} P{UAS-rnRacGAP.Zn}
Genes (11)
	Cdc42 dpp Ecol\lacZ Fas3 hep Rac1 RacGAP84C Rho1 rn Scer\GAL4 w
Insertions (9)
	P{A92}puc^E69 P{GAL4}LE P{GawB}69B P{GawB}pnr^MD237 P{GawB}ptc^559.1 P{UAS-rnRacGAP.GAP}14 P{UAS-rnRacGAP.R}7 P{UAS-rnRacGAP.R}13 P{UAS-rnRacGAP.Zn}2