Reference Report

Reference
Citation	Guillemin, K., Williams, T., Krasnow, M.A. (2001). A nuclear lamin is required for cytoplasmic organization and egg polarity in Drosophila. Nat. Cell Biol. 3(9): 848--851. (Export to RIS)
FlyBase ID	FBrf0138505
Publication Type	Research paper
PubMed ID	11533666
PubMed Abstract	Nuclear lamins are intermediate filaments that compose the nuclear lamina--the filamentous meshwork underlying the inner nuclear membrane--and are required for nuclear assembly, organization and maintenance. Here we present evidence that a nuclear lamin is also required for cytoplasmic organization in two highly polarized cell types. Zygotic loss-of-function mutations in the Drosophila gene encoding the principal lamin (Dm(0)) disrupt the directed outgrowth of cytoplasmic extensions from terminal cells of the tracheal system. Germline mutant clones disrupt dorsal-ventral polarity of the oocyte. In mutant oocytes, transcripts of the dorsal determinant Gurken, a transforming growth factor-alpha homologue, fail to localize properly around the anterodorsal surface of the oocyte nucleus; their ventral spread results in dorsalized eggs that resemble those of the classical dorsalizing mutations squid and fs(1)K10. The requirement of a nuclear lamin for cytoplasmic as well as nuclear organization has important implications for both the cellular functions of lamins and the pathogenesis of human diseases caused by lamin mutations.
DOI
Related Publication(s)
Review	Misguided and misunderstood. Anonymous, 2001, Science 293(5535): 1559 [FBrf0141789]
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Nat. Cell Biol.
Title	Nature Cell Biology
Publication Year	1999-
ISBN/ISSN	1465-7392 1476-4679
Data from Reference
Aberrations (2)
	Df(2L)cl-h3 Df(2L)GpdhA
Alleles (13)
	Ecol\lacZ^Lam-02459 fs(1)K10^LM00 Lam⁰²⁴⁵⁹ Lam⁰⁴⁶⁴³ Lam^a7 Lam^ex91 Lam^ex98 Lam^P Lam^Scer\UAS.cGa Lam^sz9 Lam^sz18 Scer\GAL4^bs.Term w^+mC
Constructs (2)
	P{bs-GAL4.Term} P{UAS-Lam.G}
Genes (7)
	Ecol\lacZ fs(1)K10 grk Lam osk Scer\GAL4 w
Insertions (5)
	P{bs-GAL4.Term}26 P{lacW}Lam^P P{PZ}Lam⁰²⁴⁵⁹ P{PZ}Lam⁰⁴⁶⁴³ P{UAS-Lam.G}6