Reference Report

Reference
Citation	Kiger, J.A., Natzle, J.E., Green, M.M. (2001). Hemocytes are essential for wing maturation in Drosophila melanogaster. Proc. Natl. Acad. Sci. U.S.A. 98(18): 10190--10195. (Export to RIS)
FlyBase ID	FBrf0138518
Publication Type	Research paper
PubMed ID	11504926
PubMed Abstract	Newly eclosed flies have wings that are highly folded and compact. Within an hour, each wing has expanded, the dorsal and ventral cuticular surfaces bonding to one another to form the mature wing. To initiate a dissection of this process, we present studies of two mutant phenotypes. First, the batone mutant blocks wing expansion, a behavior that is shown to have a mutant focus anterior to the wing in the embryonic fate map. Second, ectopic expression of protein kinase A catalytic subunit (PKAc) using certain GAL4 enhancer detector strains mimics the batone wing phenotype and also induces melanotic "tumors." Surprisingly, these GAL4 strains express GAL4 in cells, which seem to be hemocytes, found between the dorsal and ventral surfaces of newly opened wings. Ectopic expression of Ricin A in these cells reduces their number and prevents bonding of the wing surfaces without preventing wing expansion. We propose that hemocytes are present in the wing to phagocytose apoptotic epithelial cells and to synthesize an extracellular matrix that bonds the two wing surfaces together. Hemocytes are known to form melanotic tumors either as part of an innate immune response or under other abnormal conditions, including evidently ectopic PKAc expression. Ectopic expression of PKAc in the presence of the batone mutant causes dominant lethality, suggesting a functional relationship. We propose that batone is required for the release of a hormone necessary for wing expansion and tissue remodeling by hemocytes in the wing.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Aberrations (1)
	R(1)w^vC
Alleles (22)
	arm⁴ Avic\GFP^Scer\UAS.cUa bae¹ CrebB^Cbz.Scer\UAS CrebB^S162 gro¹ gro^C105 if^C.Scer\UAS nej³ nej^P nej^Q7 pan² pan³ pan^Scer\UAS.cWa pan^ΔN.Scer\UAS Pka-C1^Scer\UAS.cKa Rcom\RA^Scer\UAS.cSa Scer\GAL4^30A Scer\GAL4^71B Scer\GAL4⁶⁸⁴ Stat92E⁰⁶³⁴⁶ Stat92E^j6C8
Balancers (1)
	R(1)w^vC.ftz-lacZ
Constructs (7)
	P{Cbz} P{UAS-GFP.U} P{UAS-if.C} P{UAS-pan.dTCF} P{UAS-pan.dTCFΔN} P{UAS-Pka-C1.K} P{UAS-RA}
Genes (13)
	arm Avic\GFP bae CrebB gro if nej pan Pka-C1 Rcom\RA ru Scer\GAL4 Stat92E
Insertions (7)
	P{GAL4}684 P{GawB}30A P{GawB}71B P{lacW}nej^P P{UAS-pan.dTCF}24 P{UAS-Pka-C1.K}5.2 P{UAS-Pka-C1.K}15.3