Reference Report

Reference
Citation	Williams, J.A., Su, H.S., Bernards, A., Field, J., Sehgal, A. (2001). A circadian output in Drosophila mediated by Neurofibromatosis-1 and Ras/MAPK. Science 293(5538): 2251--2256. (Export to RIS)
FlyBase ID	FBrf0138535
Publication Type	Research paper
PubMed ID	11567138
PubMed Abstract	Output from the circadian clock controls rhythmic behavior through poorly understood mechanisms. In Drosophila, null mutations of the neurofibromatosis-1 (Nf1) gene produce abnormalities of circadian rhythms in locomotor activity. Mutant flies show normal oscillations of the clock genes period (per) and timeless (tim) and of their corresponding proteins, but altered oscillations and levels of a clock-controlled reporter. Mitogen-activated protein kinase (MAPK) activity is increased in Nf1 mutants, and the circadian phenotype is rescued by loss-of-function mutations in the Ras/MAPK pathway. Thus, Nf1 signals through Ras/MAPK in Drosophila. Immunohistochemical staining revealed a circadian oscillation of phospho-MAPK in the vicinity of nerve terminals containing pigment-dispersing factor (PDF), a secreted output from clock cells, suggesting a coupling of PDF to Ras/MAPK signaling.
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Related Publication(s)
Supplementary material	A circadian output in Drosophila mediated by Neurofibromatosis-1 and Ras/MAPK. [FBrf0141792]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Science
Title	Science
Publication Year	1895-
ISBN/ISSN	0036-8075
Data from Reference
Aberrations (1)
	Df(3R)Espl3
Alleles (23)
	dnc¹ dnc^ML Mmus\Pkaca^hs.PJ Nf1^hs.PT Nf1^P1 Nf1^P2 Nf1^{Scer\UAS.cdNa} Nf1^unspecified Pdf⁰¹ per⁰¹ phl^F22.hs Ppyr\LUC^3xCRE Ras85D^e1B RasGAP1^A13P rl^x162 Scer\GAL4^elav-C155 Scer\GAL4^P2.4.Pdf Scer\GAL4^tim.PE Sos^e2H Sos^JC2 tim⁰¹ tim^mTim7 tim^T:Ppyr\LUC
Constructs (9)
	P{3xCRE-LUC.B} P{GAL4-tim.E} P{hsNF1} P{Hsp70-Draf.gof} P{hsp70-PKA.A901} P{Pdf-GAL4.P2.4} P{Tim7} P{tim-luc.H} P{UAS-Nf1.dN}
Genes (14)
	dnc Mmus\Pkaca Nf1 Pdf per phl Ppyr\LUC Ras85D RasGAP1 rl Scer\GAL4 Sos T:Ppyr\LUC tim
Insertions (1)
	P{GawB}elav^C155