Reference Report

Reference
Citation	Castelli-Gair Hombria, J. (2001.9.19). domeless. (Export to RIS)
FlyBase ID	FBrf0138617
Publication Type	Personal communication to FlyBase
PubMed ID
PubMed Abstract
Text of Personal Communication	From jec24@XXXXXXXXXXXXXXX Wed Sep 19 15:47:28 2001 To: Rachel Drysdale <rd120@XXXXXXXXXXXXXXX> Subject: dome Dear Rachel, This info relates to domeless (dome). Not all of this will be published in our Current Biology paper. dome is the computer annotated GADFLY gene CG14226. We isolated the dome alleles from the Gottingen X P collection. We named all alleles using as superscript the original number used in the Gottingen X P collection (i.e.dome³⁶⁷ corresponds to l(1)G0367). By plasmid rescue we found that seven elements are inserted 400bp apart in 18E. l(1)G0321 is inserted 30bp upstream the start of LD32858 and has no zygotic nor maternal cuticle phenotype. The other rescued P elements are inserted in the 5' UTR at the following positions with respect to the start of LD32858: dome⁴⁰⁵ is inserted at 20b, dome³⁶⁷ 77 b, dome²¹⁷ and dome²¹⁸ 154b, dome⁴⁶⁸ 268 and dome⁴⁴¹ 376b. We have other dome alleles that have not been rescued: l(1)G0199b, l(1)G0264 and l(1)G0282. 321, 405 and 367 are hypomorphic insertions, the others have strong zygotic domeless phenotypes. Based on the identical phenotypes in germ line clones of the strong alleles to germ line clones of stat92E null alleles, they may be null. There is a further strong allele: dome⁹ that was isolated from line l(1)G0009. This is not caused by the P insertion which is in Act5C as the phenotype can be separated from the P insertion. We know that all the above mutants are dome alleles because the zygotic spiracle phenotypes are rescued by UAS-dome5.1. (see below). UAS constructs: The EST LD32858 was obtained from Research Genetics from the embryonic 0-22 hours LD library. UAS-dome was made by an EcoR1/Xho1 excision from pOT2a and directionally subcloned into EcoR1/Sal1 cut pUAST. UAS-dome^ΔTMCYT and UAS-dome^ΔCYT were similarly subcloned but using the Bgl2 and EcoRV sites within dome. Several independent lines were tested for each experiment. One of the UAS-dome constructs (UAS-dome5.1) rescues the spiracle phenotype even in the absence of a GAL4 driver line, suggesting that a nearby enhancer activates the construct in the posterior spiracles. James
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type
Abbreviation
Title
ISBN/ISSN
Data from Reference
Alleles (17)
	Act5C^G0009 dome^5.1 dome^G0009 dome^G0199b dome^G0217 dome^G0218 dome^G0264 dome^G0282 dome^G0321 dome^G0367 dome^G0405 dome^G0441 dome^G0468 dome^{Scer\UAS.cCGHa} dome^{ΔCYT.Scer\UAS} dome^{ΔTMCYT.Scer\UAS} w^+mC
Constructs (3)
	P{UAS-dome.CGH} P{UAS-dome.ΔCYT} P{UAS-dome.ΔTMCYT}
Genes (3)
	Act5C dome w
Insertions (12)
	P{lacW}Act5C^G0009 P{lacW}dome^G0199b P{lacW}dome^G0217 P{lacW}dome^G0218 P{lacW}dome^G0264 P{lacW}dome^G0282 P{lacW}dome^G0321 P{lacW}dome^G0367 P{lacW}dome^G0405 P{lacW}dome^G0441 P{lacW}dome^G0468 P{UAS-dome.CGH}5.1