Reference Report

Reference
Citation	Schaefer, M., Petronczki, M., Dorner, D., Forte, M., Knoblich, J.A. (2001). Heterotrimeric G proteins direct two modes of asymmetric cell division in the Drosophila nervous system. Cell 107(2): 183--194. (Export to RIS)
FlyBase ID	FBrf0139609
Publication Type	Research paper
PubMed ID	11672526
PubMed Abstract	In Drosophila, distinct mechanisms orient asymmetric cell division along the apical-basal axis in neuroblasts and along the anterior-posterior axis in sensory organ precursor (SOP) cells. Here, we show that heterotrimeric G proteins are essential for asymmetric cell division in both cell types. The G protein subunit G(alpha)i localizes apically in neuroblasts and anteriorly in SOP cells before and during mitosis. Interfering with G protein function by G(alpha)i overexpression or depletion of heterotrimeric G protein complexes causes defects in spindle orientation and asymmetric localization of determinants. G(alpha)i is colocalized and associated with Pins, a protein that induces the release of the betagamma subunit and might act as a receptor-independent G protein activator. Thus, asymmetric activation of heterotrimeric G proteins by a receptor-independent mechanism may orient asymmetric cell divisions in different cell types.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Cell
Title	Cell
Publication Year	1974-
ISBN/ISSN	0092-8674
Data from Reference
Aberrations (1)
	Df(2R)P72
Alleles (15)
	cta¹ fz²⁵ Gαi^{dsRNA.Scer\UAS} Gαi^{Q205L.Scer\UAS} Gαi^Scer\UAS.cLa Gβ13F^+tSa Gβ13F^EP1071 Gβ13F^Δ1-96A insc^{Cen.Scer\UAS.T:Ecol\lacZ} insc^hs.PKb raps⁸³ raps^Δ50 Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} Scer\GAL4^sca-109-68 w^+mC
Constructs (7)
	P{Gβ13F^+tSa} P{hs-insc.Kb} P{matα4-GAL-VP16} P{UAS-G-iα65A.L} P{UAS-G-iα65A.Q205L} P{UAS-G-iα65A.RNAi} P{UAS-insc.Cen.T:lacZ}
Genes (20)
	ase baz cta fz Gapdh2 Gαi Gαo Gβ13F insc mira Myb numb raps Scer\GAL4 T:Ecol\lacZ T:Hsim\VP16 w α-Spec γTub23C γTub37C
Insertions (2)
	P{EP}Gβ13F^EP1071 P{GawB}sca^109-68