Reference Report

Reference
Citation	Yoshida, H., Inoue, Y.H., Hirose, F., Sakaguchi, K., Matsukage, A., Yamaguchi, M. (2001). Over-expression of DREF in the Drosophila wing imaginal disc induces apoptosis and a notching wing phenotype. Genes Cells 6(10): 877--886. (Export to RIS)
FlyBase ID	FBrf0139673
Publication Type	Research paper
PubMed ID	11683916
PubMed Abstract	DNA replication-related element binding factor (DREF) has been suggested to be involved in regulation of DNA replication- and proliferation-related genes in Drosophila. While the effects on the mutation in the DNA replication-related element (DRE) in cultured cells have been studied extensively, the consequences of elevating wild-type DREF activity in developing tissues have hitherto remained unclear.We over-expressed DREF in the wing imaginal disc using a GAL4-UAS targeted expression system in Drosophila. Over-expression of DREF induced a notching wing phenotype, which was associated with ectopic apoptosis. A half reduction of the reaper, head involution defective and grim gene dose suppressed this DREF-induced notching wing phenotype. Furthermore, this was also the case with co-expression of baculovirus P35, a caspase inhibitor. In addition, over-expression of the 32 kDa boundary element-associated factor (BEAF-32), thought to compete against DREF for common binding sites in genomic regions, rescued the DREF-induced notching wing phenotype, while a half reduction of the genomic region, including the BEAF-32 gene, exerted enhancing effects. To our knowledge, this is the first evidence for a genetic interaction between DREF and BEAF-32.The DREF-induced notching wing phenotype is caused by induction of apoptosis in the Drosophila wing imaginal disc.
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genes Cells
Title	Genes to cells : devoted to molecular & cellular mechanisms
Publication Year	1996-
ISBN/ISSN	1356-9597
Data from Reference
Aberrations (2)
	Df(2R)Jp1 Df(3L)H99
Alleles (26)
	BacA\p35^Scer\UAS.cHa BEAF-32^Scer\UAS.cYa Dref^Scer\UAS.cSa E2f⁹¹ Scer\GAL4^32B Scer\GAL4^55B Scer\GAL4¹⁸⁵⁴ Scer\GAL4¹⁸⁸² Scer\GAL4²⁶⁸⁹ Scer\GAL4³⁷³⁵ Scer\GAL4⁴⁹³⁵ Scer\GAL4^Act88F.PD Scer\GAL4^ap-md544 Scer\GAL4^Cg.PA Scer\GAL4^Dll-md23 Scer\GAL4^dpp.blk1 Scer\GAL4^elav.PLu Scer\GAL4^en-e16E Scer\GAL4^insc-Mz1407 Scer\GAL4^l(1)BP-1 Scer\GAL4^nos.PG Scer\GAL4^prd.RG1 Scer\GAL4^ptc-559.1 Scer\GAL4^rho.PL Scer\GAL4^twi.PG Scer\GAL4^wg.PM
Constructs (12)
	P{Act88F-GAL4.D} P{Cg-GAL4.A} P{GAL4-dpp.blk1} P{GAL4-elav.L} P{GAL4-nos.NGT} P{GAL4-prd.F} P{GAL4-twi.G} P{GAL4-ve.L} P{GAL4-wg.M} P{UAS-BEAF-32.Y} P{UAS-Dref.S} P{UAS-p35.H}
Genes (5)
	BacA\p35 BEAF-32 Dref E2f Scer\GAL4
Insertions (20)
	P{en2.4-GAL4}e16E P{GAL4}1854 P{GAL4}1882 P{GAL4}2689 P{GAL4}3735 P{GAL4}4935 P{GawB}32B P{GawB}ap^md544 P{GawB}Dll^md23 P{GawB}Eip63F-1^55B P{GawB}insc^Mz1407 P{GawB}l(1)BP¹ P{GawB}ptc^559.1 P{UAS-Dref.S}12 P{UAS-Dref.S}30 P{UAS-Dref.S}40 P{UAS-Dref.S}41 P{UAS-Dref.S}42 P{UAS-Dref.S}51 P{UAS-Dref.S}82
Transcripts (1)
	Scer\GAL4^Cg.PARA