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Citation
Park, J.M., Werner, J., Kim, J.M., Lis, J.T., Kim, Y.J. (2001). Mediator, not holoenzyme, is directly recruited to the heat shock promoter by HSF upon heat shock.  Mol. Cell 8(1): 9--19.
FlyBase ID
FBrf0139821
Publication Type
Research paper
Abstract

Activators of RNA polymerase II (Pol II) transcription have been shown to bind several coactivators and basal factors in vitro. Whether such interactions play a primary regulatory role in recruiting these factors to activator-associated chromosomal target sites in living cells remains unclear. Here, we show that upon heat shock the Pol II-free form of Mediator is rapidly recruited to HSF binding sites. Unlike the TAFs and Pol II, the interaction between Mediator and HSF on chromosomal loci is direct and mechanistically separable from the preinitiation complex assembly step. Therefore, the activator-Mediator interaction likely underlies the initiation of signal transfer from enhancer-bound activators to the basal transcription machinery.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Mol. Cell
    Title
    Molecular Cell
    Publication Year
    1997-
    ISBN/ISSN
    1097-2765 1097-4164
    Data From Reference
    Alleles (2)
    Gene Groups (1)
    Genes (22)
    Insertions (2)
    Transgenic Constructs (1)