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Manning, G. (2001.10.15). Novel kinase: WSCK. 
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Date: Mon, 15 Oct 2001  14:58:15  \-0700
To: Gillian Millburn (Genetics) <gm119@XXXX>
From: Gerard Manning <gerard-manning@XXXX>
Subject: Novel kinase: WSCK
WSCK is a predicted novel receptor tyrosine kinase. It was discovered in a
computational screen for kinase domains in fly genomic sequence; the
initial HMM hit was extended using Genewise gene finding and overlapping
ESTs, giving a full open reading frame ((TrEMBL: P83097) and flanking UTRs
. The 2980nt predicted transcript encodes an 809 AA protein with 5' and 3'
UTRs and a polyA tail, if a single base is inserted into the genomic
sequence to correct a predicted frameshift.
The sequence predicts a receptor-like protein, with a signal peptide from
1-32 and a single transmembrane domain in the region of 418-463 (different
methods predict different co-ordinates within that region). The putative
extracellular region includes a WSC domain from 42-115, a full fibronectin
type III (FnIII) repeat from 129-233 and a partial FnIII repeat from
248-286. The WSC domain has been weakly ascribed a role in carbohydrate
binding. FnIII domains are common in extracellular regions of cell surface
proteins, especially receptor tyrosine kinases and cytokine receptors and
may bind heparin or other cell surface proteins.
The putative intracellular region has a divergent kinase domain from
511-770 which is most similar to tyrosine kinase domains from the tie/tek
and FGF-R families of receptor tyrosine kinases, though the similarity of
kinases within these families is much higher to each other than to WSCK.
The divergence of this sequence cautions against strong conclusions from
the homology or that this is a fully functional kinase domain. However, the
relationship to FGF receptor kinases may be relevant to the carbohydrate
and heparin-binding properties of the WSC and FnIII domains, given that FGF
binding to receptor is dependent on heparin binding; WSCK could
speculatively be a FGF co-receptor.
The sequence is almost completely covered by 15 ESTs (excluding the
frameshift region), from a variety of libraries: 5 from embryo (LD
library), 5 from head (1 from GH library, 4 from RH normalized head
library), 1 from ovary (GM), 1 from larval/pupal stages (LP) and three from
S2 cells (SD library).
The sequence maps as a two-exon transcript to 96A13-16, between predicted
gene CG6668, a conserved novel protein, and Syx 18, encoding a t-SNARE.
\-Gerard Manning.
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