Reference Report

Reference
Citation	Lai, E.C., Deblandre, G.A., Kintner, C., Rubin, G.M. (2001). Drosophila neuralized is a ubiquitin ligase that promotes the internalization and degradation of delta. Dev. Cell 1(6): 783--794. (Export to RIS)
FlyBase ID	FBrf0141465
Publication Type	Research paper
PubMed ID	11740940
PubMed Abstract	The Drosophila gene neuralized (neur) has long been recognized to be essential for the proper execution of a wide variety of processes mediated by the Notch (N) pathway, but its role in the pathway has been elusive. In this report, we present genetic and biochemical evidence that Neur is a RING-type, E3 ubiquitin ligase. Next, we show that neur is required for proper internalization of Dl in the developing eye. Finally, we demonstrate that ectopic Neur targets Dl for internalization and degradation in a RING finger-dependent manner, and that the two exist in a physical complex. Collectively, our data indicate that Neur is a ubiquitin ligase that positively regulates the N pathway by promoting the endocytosis and degradation of Dl.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Aberrations (1)
	Df(3R)gro^b32.2
Alleles (21)
	Avic\GFP^{Stinger.Scer\UAS.T:nls-tra} Dl^9P Dl^hs.PS eff^s81 eff^s278 eff^s1782 gro^+t10.4 gro^b32.2 N^Hsp70Bb.PL neur¹¹ neur^A101 neur^{RING.Scer\UAS} neur^Scer\UAS.cLa neur^{Scer\UAS.L.T:Hsap\MYC} neur^{αRING.Scer\UAS} Prosβ6^1.Scer\UAS Scer\GAL4^Bx-MS1096 Scer\GAL4^dpp.blk1 Scer\GAL4^GMR.PF Scer\GAL4^sca-537.4 sty^{Scer\UAS.T:Hsap\MYC}
Constructs (12)
	P{GAL4-dpp.blk1} P{GAL4-ninaE.GMR} P{hsN} P{hsp70-Dl.S} P{Stinger} P{UAS-DTS5.S} P{UAS-neur.L.Myc} P{UAS-neur.L} P{UAS-neur.RING} P{UAS-neur.αRING} P{UAS-Stinger} P{UAS-sty.MYC}
Genes (10)
	Avic\GFP Dl eff gro N neur Prosβ6 Scer\GAL4 sty T:Hsap\MYC
Insertions (3)
	P{GawB}Bx^MS1096 P{GawB}sca^537.4 P{lArB}neur^A101