Reference Report

Reference
Citation	Jin, J., Anthopoulos, N., Wetsch, B., Binari, R.C., Isaac, D.D., Andrew, D.J., Woodgett, J.R., Manoukian, A.S. (2001). Regulation of Drosophila tracheal system development by protein kinase B. Dev. Cell 1(6): 817--827. (Export to RIS)
FlyBase ID	FBrf0141466
Publication Type	Research paper
PubMed ID	11740943
PubMed Abstract	Protein kinase B (PKB, also termed Akt) is a phosphatidylinositol 3' kinase (PI3'K)-dependent enzyme implicated in survival signaling and human tumorigenesis. To identify potential targets of this protein kinase, we employed a genetic screen in Drosophila. Among several genes that genetically interacted with PKB was trachealess (trh), which encodes a bHLH-PAS domain transcription factor required for development of the trachea and other tubular organs. Trh activates expression of the fibroblast growth factor receptor Breathless, which, in turn, is required for directed migration of all tracheal branches. Using a combination of biochemical and transgenic approaches, we show that direct phosphorylation of Trh by PKB at serine 665 is essential for nuclear localization and functional activation of this regulator of branching morphogenesis.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Aberrations (3)
	Df(2L)Pi3K21B-A Df(2L)Pi3K21B-B Df(3R)sbd45
Alleles (17)
	Akt1¹ Akt1⁰⁴²²⁶ Ecol\lacZ^btl.B123 Ppyr\LUC^btl.B123 Pten^c494 Pten^Scer\UAS.cSa Scer\GAL4^arm.PS trh¹ trh² trh¹⁰⁵¹² trh^{Act5C.T:Zzzz\FLAG} trh^{S571A.Act5C.T:Zzzz\FLAG} trh^{S665A.Act5C.T:Zzzz\FLAG} trh^{S665A.Scer\UAS.T:Zzzz\FLAG} trh^{S665D.Act5C.T:Zzzz\FLAG} trh^{S665D.Scer\UAS.T:Zzzz\FLAG} trh^{Scer\UAS.T:Zzzz\FLAG}
Constructs (5)
	P{GAL4-arm.S} P{UAS-Pten.S} P{UAS-trh.FLAG} P{UAS-trh.S665A.FLAG} P{UAS-trh.S665D.FLAG}
Genes (8)
	Akt1 btl Pi3K21B Ppyr\LUC Pten Scer\GAL4 T:Zzzz\FLAG trh
Transcripts (1)
	Akt1^P1627RA