Reference Report

Reference
Citation	Kumar, J.P., Wilkie, G.S., Tekotte, H., Moses, K., Davis, I. (2001). Perturbing nuclear transport in Drosophila eye imaginal discs causes specific cell adhesion and axon guidance defects. Dev. Biol. 240(2): 315--325. (Export to RIS)
FlyBase ID	FBrf0141476
Publication Type	Research paper
PubMed ID	11784066
PubMed Abstract	To study nucleocytoplasmic transport during multicellular development, we developed a sensitive nuclear protein import assay in living blastoderm embryos. We show that dominant negative truncations of the human nuclear transport receptor karyopherinbeta/Importinbeta (DNImpbeta) disrupt mRNA export and protein import in Drosophila. To test the sensitivity of different developmental processes to nuclear trafficking perturbations, we expressed DNImpbeta behind the morphogenetic furrow of the eye disc, at a time when photoreceptors are patterned and project their axons to the brain. DNImpbeta expression does not disrupt the correct specification of different photoreceptors, but causes a defect in cell adhesion that leads to some photoreceptors descending below the layer of ommatidia. The photoreceptors initially project their axons correctly to the posterior, but later their axons are unable to enter the optic stalk en route to the brain and continue to project an extensive network of misguided axons. The axon guidance and cell adhesion defects are both due to a disruption in the function of Ketel, the Drosophila ortholog of Importinbeta. We conclude that cell adhesion and axon guidance in the eye have specific requirements for nucleocytoplasmic transport, despite involving processes that occur primarily at the cell surface.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (3)
	Df(2L)pr76 Df(2L)TW2 Df(2L)TW161
Alleles (16)
	arm³ arm⁴ arm^Scer\UAS.wt arm^unspecified Avic\GFP^{Ubi-p63E.T:Ecol\lacZ,T:Zzzz\nls1} Fs(2)Ket^RE28 Fs(2)Ket^RE31 Fs(2)Ket^RE34 Hsap\KPNB1^Scer\UAS.cKa Scer\GAL4^ey.PH Scer\GAL4^GMR.12 Scer\GAL4^GMR.15 Scer\GAL4^hs.2sev Scer\GAL4^ptc-559.1 Scer\GAL4^sca-537.4 Scer\GAL4^sd-SG29.1
Constructs (7)
	P{GAL4-ey.H} P{GAL4-Hsp70.sev} P{GMR-GAL4.12} P{GMR-GAL4.15} P{UAS-arm.wt} P{UAS-HsImp.β45-462} P{Ubi-GFP.nls}
Genes (15)
	arm ato Avic\GFP boss elav Fs(2)Ket futsch GMR hb Hsap\KPNB1 repo run Scer\GAL4 T:Ecol\lacZ T:Zzzz\nls1
Insertions (3)
	P{GawB}ptc^559.1 P{GawB}sca^537.4 P{GawB}sd^SG29.1