Reference Report

Reference
Citation	Rintelen, F., Stocker, H., Thomas, G., Hafen, E. (2001). PDK1 regulates growth through Akt and S6K in Drosophila. Proc. Natl. Acad. Sci. U.S.A. 98(26): 15020--15025. (Export to RIS)
FlyBase ID	FBrf0141724
Publication Type	Research paper
PubMed ID	11752451
PubMed Abstract	The insulin/insulin-like growth factor-1 signaling pathway promotes growth in invertebrates and vertebrates by increasing the levels of phosphatidylinositol 3,4,5-triphosphate through the activation of p110 phosphatidylinositol 3-kinase. Two key effectors of this pathway are the phosphoinositide-dependent protein kinase 1 (PDK1) and Akt/PKB. Although genetic analysis in Caenorhabditis elegans has implicated Akt as the only relevant PDK1 substrate, cell culture studies have suggested that PDK1 has additional targets. Here we show that, in Drosophila, dPDK1 controls cellular and organism growth by activating dAkt and S6 kinase, dS6K. Furthermore, dPDK1 genetically interacts with dRSK but not with dPKN, encoding two substrates of PDK1 in vitro. Thus, the results suggest that dPDK1 is required for dRSK but not dPKN activation and that it regulates insulin-mediated growth through two main effector branches, dAkt and dS6K.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (27)
	Akt1¹ Akt1^Scer\UAS.cRa InR^Scer\UAS.cHa Pdk1² Pdk1³ Pdk1⁴ Pdk1^4ΔEP Pdk1⁵ Pdk1^5ΔEP Pdk1^{A467V.Scer\UAS} Pdk1^EP837 Pdk1^EP3091 Pdk1^EP3644 Pdk1^Scer\UAS.cRa Pdk1^unspecified Pkn^Scer\UAS.cRa Pten^c494 Pten^dj189 S6k^l-1 S6k^Scer\UAS.cMa S6kII^Scer\UAS.cRa Scer\GAL4^ap-md544 Scer\GAL4^arm.PS Scer\GAL4^GMR.PB Scer\GAL4^GMR.PF Scer\GAL4^Scer\FRT.GMR w^+mC
Constructs (11)
	P{GAL4-arm.S} P{GAL4-ninaE.GMR} P{GMR-GAL4.FRT} P{GMR-GAL4(FRT-)} P{UAS-Akt1.R} P{UAS-InR.H} P{UAS-Pdk1.A467V} P{UAS-Pdk1.R} P{UAS-Pkn.R} P{UAS-S6k.M} P{UAS-S6kII.R}
Genes (11)
	Akt1 GMR InR Pdk1 Pi3K92E Pkn Pten S6k S6kII Scer\GAL4 w
Insertions (4)
	P{EP}Pdk1^EP837 P{EP}Pdk1^EP3091 P{EP}Pdk1^EP3644 P{GawB}ap^md544