Reference Report

Reference
Citation	Morin, X., Daneman, R., Zavortink, M., Chia, W. (2001). A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila. Proc. Natl. Acad. Sci. U.S.A. 98(26): 15050--15055. (Export to RIS)
FlyBase ID	FBrf0141725
Publication Type	Research paper
PubMed ID	11742088
PubMed Abstract	In Drosophila, enhancer trap strategies allow rapid access to expression patterns, molecular data, and mutations in trapped genes. However, they do not give any information at the protein level, e.g., about the protein subcellular localization. Using the green fluorescent protein (GFP) as a mobile artificial exon carried by a transposable P-element, we have developed a protein trap system. We screened for individual flies, in which GFP tags full-length endogenous proteins expressed from their endogenous locus, allowing us to observe their cellular and subcellular distribution. GFP fusions are targeted to virtually any compartment of the cell. In the case of insertions in previously known genes, we observe that the subcellular localization of the fusion protein corresponds to the described distribution of the endogenous protein. The artificial GFP exon does not disturb upstream and downstream splicing events. Many insertions correspond to genes not predicted by the Drosophila Genome Project. Our results show the feasibility of a protein trap in Drosophila. GFP reveals in real time the dynamics of protein's distribution in the whole, live organism and provides useful markers for a number of cellular structures and compartments.
DOI
Related Publication(s)
Personal communication to FlyBase	GFP Protein Trap Insertion Lines. Cooley et al., 2002.10-2003.5, GFP Protein Trap Insertion Lines. [FBrf0161605] Helping FlyBase: PTT lines. Morin, 2003.6.19, Helping FlyBase: PTT lines. [FBrf0159882]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (108)
	Atpα^G00109 Avic\GFP^Atpα-G00109 Avic\GFP^Bsg-G00413 Avic\GFP^bun-G409 Avic\GFP^{CG1640-G00282} Avic\GFP^CG2556-G365 Avic\GFP^{CG6084-G00112} Avic\GFP^CG6783-G88 Avic\GFP^CG9894-G180 Avic\GFP^CG17273-G245 Avic\GFP^Clic-G00264 Avic\GFP^clu-G00271 Avic\GFP^Dek-G119 Avic\GFP^Df31-G196 Avic\GFP^eIF-4a-G29 Avic\GFP^G50 Avic\GFP^G108 Avic\GFP^G116 Avic\GFP^G123 Avic\GFP^G145 Avic\GFP^G154 Avic\GFP^G157 Avic\GFP^G214 Avic\GFP^G215 Avic\GFP^G231 Avic\GFP^G258 Avic\GFP^G260 Avic\GFP^G270 Avic\GFP^G276 Avic\GFP^G281 Avic\GFP^G284 Avic\GFP^G287 Avic\GFP^G289 Avic\GFP^G304 Avic\GFP^G318 Avic\GFP^G341 Avic\GFP^G357 Avic\GFP^G357bis Avic\GFP^G360 Avic\GFP^G361 Avic\GFP^G370 Avic\GFP^G377 Avic\GFP^G392 Avic\GFP^G419 Avic\GFP^G428 Avic\GFP^{GA.T:Zzzz\His6} Avic\GFP^{GB.T:Zzzz\His6} Avic\GFP^{GC.T:Zzzz\His6} Avic\GFP^gish-G38 Avic\GFP^{G-oα47A-G430} Avic\GFP^His2Av-G280 Avic\GFP^{Jupiter-G00147} Avic\GFP^Karyβ3-G169 Avic\GFP^Lac-G00044 Avic\GFP^LamC-G00158 Avic\GFP^Lam-G262 Avic\GFP^Msp-300-G129 Avic\GFP^Nrg-G00305 Avic\GFP^nrv2-G74 Avic\GFP^Pdcd4-G93 Avic\GFP^Pdi-G00198 Avic\GFP^Rtnl1-G9 Avic\GFP^sgg-G33 Avic\GFP^sgg-G138 Avic\GFP^sgl-G126 Avic\GFP^sls-G53 Avic\GFP^Tm1-G5 Avic\GFP^tral-G89 Avic\GFP^Vha16-G7 Avic\GFP^{VhaSFD-G00259} Avic\GFP^vkg-G454 Avic\GFP^{Zasp52-G00189} Bacc^G180 bun^G409 CG1640^G00282 CG2556^G365 CG6084^G00112 CG17273^G245 Clic^G00264 clu^G00271 Dek^G119 Df31^G196 eIF-4a^G29 fabp^G88 gish^G38 Gαo^G430 His2Av^G280 Jupiter^G00147 Karyβ3^G169 Lac^G00044 Lam^G262 LamC^G00158 Msp-300^G129 Nrg^G00305 nrv2^G74 Pdcd4^G93 Pdi^G00198 Rtnl1^G9 sgg^G33 sgg^G138 sgl^G126 sls^G53 Tm1^G5 tral^G89 Vha16-1^G7 VhaSFD^G00259 vkg^G454 Zasp52^G00189
Constructs (4)
	P{PTT-GA} P{PTT-GB} P{PTT-GC} P{PTT-un1}
Genes (39)
	Atpα Avic\GFP Bacc bun CG1640 CG2556 CG6084 CG17273 Clic clu Dek Df31 eIF-4a fabp gish Gαo His2Av Jupiter Karyβ3 Lac Lam LamC Msp-300 Nrg nrv2 Pdcd4 Pdi Rtnl1 sgg sgl sls T:Zzzz\His6 Tm1 tral tun Vha16-1 VhaSFD vkg Zasp52
Insertions (77)
	P{PTT-GA}Bsg^G00413 P{PTT-GA}clu^G00271 P{PTT-GA}Dek^G119 P{PTT-GA}fabp^G88 P{PTT-GA}G116 P{PTT-GA}G157 P{PTT-GA}G258 P{PTT-GA}G270 P{PTT-GA}Gαo^G430 P{PTT-GA}III-1b P{PTT-GA}Jupiter^G00147 P{PTT-GA}Msp-300^G129 P{PTT-GA}Nrg^G00305 P{PTT-GA}Pdi^G00198 P{PTT-GA}Rtnl1^G9 P{PTT-GA}sgg^G138 P{PTT-GA}tral^G89 P{PTT-GA}Vha16-1^G7 P{PTT-GA}VhaSFD^G00259 P{PTT-GB}bun^G409 P{PTT-GB}Df31^G196 P{PTT-GB}G145 P{PTT-GB}G154 P{PTT-GB}G215 P{PTT-GB}G260 P{PTT-GB}G276 P{PTT-GB}G281 P{PTT-GB}G284 P{PTT-GB}G287 P{PTT-GB}G304 P{PTT-GB}G318 P{PTT-GB}G357 P{PTT-GB}G361 P{PTT-GB}G377 P{PTT-GB}III-3a P{PTT-GB}III-3b P{PTT-GB}III-5 P{PTT-GB}Lam^G262 P{PTT-GB}LamC^G00158 P{PTT-GB}Pdcd4^G93 P{PTT-GB}sgl^G126 P{PTT-GB}Zasp52^G00189 P{PTT-GC}Atpα^G00109 P{PTT-GC}Bacc^G180 P{PTT-GC}CG1640^G00282 P{PTT-GC}CG2556^G365 P{PTT-GC}CG6084^G00112 P{PTT-GC}Clic^G00264 P{PTT-GC}G108 P{PTT-GC}G214 P{PTT-GC}G231 P{PTT-GC}G392 P{PTT-GC}G419 P{PTT-GC}His2Av^G280 P{PTT-GC}III-1 P{PTT-GC}III-3 P{PTT-GC}III-4a P{PTT-GC}III-4b P{PTT-GC}III-5 P{PTT-GC}Tm1^G5 P{PTT-un1}CG17273^G245 P{PTT-un1}eIF-4a^G29 P{PTT-un1}G50 P{PTT-un1}G123 P{PTT-un1}G289 P{PTT-un1}G341 P{PTT-un1}G357bis P{PTT-un1}G360 P{PTT-un1}G370 P{PTT-un1}G428 P{PTT-un1}gish^G38 P{PTT-un1}Karyβ3^G169 P{PTT-un1}Lac^G00044 P{PTT-un1}nrv2^G74 P{PTT-un1}sgg^G33 P{PTT-un1}sls^G53 P{PTT-un1}vkg^G454
Transcripts (14)
	Avic\GFP^CG9894-G180RA Avic\GFP^Dek-G119RA Avic\GFP^gish-G38RA Avic\GFP^His2Av-G280RA Avic\GFP^{Jupiter-G00147}RA Avic\GFP^LamC-G00158RA Avic\GFP^Lam-G262RA Avic\GFP^Msp-300-G129RA Avic\GFP^Rtnl1-G9RA Avic\GFP^sgg-G138RA Avic\GFP^sls-G53RA Avic\GFP^Tm1-G5RA Avic\GFP^tral-G89RA Avic\GFP^vkg-G454RA