Reference
Citation
Morin, X., Daneman, R., Zavortink, M., Chia, W. (2001). A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila.  Proc. Natl. Acad. Sci. U.S.A. 98(26): 15050--15055.
FlyBase ID
FBrf0141725
Publication Type
Research paper
Abstract
In Drosophila, enhancer trap strategies allow rapid access to expression patterns, molecular data, and mutations in trapped genes. However, they do not give any information at the protein level, e.g., about the protein subcellular localization. Using the green fluorescent protein (GFP) as a mobile artificial exon carried by a transposable P-element, we have developed a protein trap system. We screened for individual flies, in which GFP tags full-length endogenous proteins expressed from their endogenous locus, allowing us to observe their cellular and subcellular distribution. GFP fusions are targeted to virtually any compartment of the cell. In the case of insertions in previously known genes, we observe that the subcellular localization of the fusion protein corresponds to the described distribution of the endogenous protein. The artificial GFP exon does not disturb upstream and downstream splicing events. Many insertions correspond to genes not predicted by the Drosophila Genome Project. Our results show the feasibility of a protein trap in Drosophila. GFP reveals in real time the dynamics of protein's distribution in the whole, live organism and provides useful markers for a number of cellular structures and compartments.
PubMed ID
PubMed Central ID
PMC64981 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase
GFP Protein Trap Insertion Lines.
Cooley et al., 2002.10-2003.5, GFP Protein Trap Insertion Lines. [FBrf0161605]
FlyTrap insertion lines - genomic sequence locations.
Levis et al., 2013-, FlyTrap insertion lines - genomic sequence locations. [FBrf0222141]
Helping FlyBase: PTT lines.
Morin, 2003.6.19, Helping FlyBase: PTT lines. [FBrf0159882]
Associated Information
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Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Proc. Natl. Acad. Sci. U.S.A.
    Title
    Proceedings of the National Academy of Sciences of the United States of America
    Publication Year
    1915-
    ISBN/ISSN
    0027-8424
    Data From Reference
    Alleles (106)
    Genes (38)
    Datasets (1)
    Insertions (77)
    Experimental Tools (2)
    Transgenic Constructs (4)
    Transcripts (14)