Open Close
Reference
Citation
Goto, A. (2001.12.6). P{Hml-GAL4.G} construct and insertions. 
FlyBase ID
FBrf0141807
Publication Type
Personal communication to FlyBase
Abstract
PubMed ID
PubMed Central ID
Text of Personal Communication
Date: Fri, 07 Dec 2001  08:46:48  \-0500
To: Gillian Millburn (Genetics) <gm119@XXXX>
From: Kevin Cook <kcook@XXXX>
Subject: P{Hml-GAL4.G} construct and insertions
The following information accompanied stocks donated to the Bloomington
Stock Center by Akira Goto, Nagoya University (11/01).
The identification and characterization of Hml was described in Goto et al.
2001, Biochem J. 359: 99-108 <up>FBrf0138238</up>.
pP{Hml-GAL4.G} was constructed by combining a GAL4-spanning BamHI-NotI
fragment excised from pGaTB and the 3.0 kb upstream promoter region of Hml
and cloning them into pP{CaSpeR-4}. The promoter region of Hml flanked by
BamHI sites was prepared by amplification of genomic DNA using primers
5'-CGCGGATCCAAGAACTTTACATAAATTCCGGTATC-3' and
5'-CGCGGATCCTTTGTTAGGCTAATCGGAAATTGTTGG-3' based on the sequence nt
20501-23450 in GenBank AC015143.
Insertions of P{Hml-GAL4.G} include the following:
P{Hml-GAL4.G}5-6 homozygous viable and fertile chromosome 3 insertion
P{Hml-GAL4.G}6-4 homozygous viable and fertile chromosome 2 insertion
P{Hml-GAL4.G}8-5 homozygous viable and fertile chromosome 3 insertion
P{Hml-GAL4.G}13-6 homozygous viable and fertile chromosome 3 insertion
Endogenous Hml is expressed at low levels in larval hemocytes. A second
chromosome with P{Hml-GAL4.G}6-4, P{UAS- GFP::lacZ.nls } and P{UAS-GFP.S65T}
insertions gave weak but detectable GFP expression when heterozygous, and
strong GFP expression when homozygous. GFP expression was not detectable
when a P{Hml-GAL4.G} insertion was crossed to single UAS-GFP construct
insertions.
__________________________________________________________
Kevin Cook, Ph.D. Bloomington Drosophila Stock Center
Department of Biology http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University 812-855-5782
1001 E. Third St. 812-855-2577 (fax)
Bloomington, IN 47405-3700 kcook@XXXX
DOI
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Abbreviation
    Title
    ISBN/ISSN
    Data From Reference
    Alleles (3)
    Genes (4)
    Insertions (4)
    Experimental Tools (1)
    Transgenic Constructs (3)