Reference Report

Reference
Citation	Pavlopoulos, E., Pitsouli, C., Klueg, K.M., Muskavitch, M.A., Moschonas, N.K., Delidakis, C. (2001). neuralized encodes a peripheral membrane protein involved in delta signaling and endocytosis. Dev. Cell 1(6): 807--816. (Export to RIS)
FlyBase ID	FBrf0144791
Publication Type	Research paper
PubMed ID	11740942
PubMed Abstract	Activation of the Notch (N) receptor involves an intracellular proteolytic step triggered by shedding of the extracellular N domain (N-EC) upon ligand interaction. The ligand Dl has been proposed to effect this N-EC shedding by transendocytosing the latter into the signal-emitting cell. We find that Dl endocytosis and N signaling are greatly stimulated by expression of neuralized (neur). neur inactivation suppresses Dl endocytosis, while its overexpression enhances Dl endocytosis and Notch-dependent signaling. We show that neur encodes an intracellular peripheral membrane protein. Its C-terminal RING domain is necessary for Dl accumulation in endosomes, but may be dispensable for Dl signaling. The potent modulatory effect of Neur on Dl activity makes Neur a candidate for establishing signaling asymmetries within cellular equivalence groups.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Aberrations (1)
	Df(3R)gro^b32.2
Alleles (15)
	Dl^RevF10 Dl^Scer\UAS.cLa Ecol\lacZ^Dl-A326.2F3 Ecol\lacZ^neur-A101 Ecol\lacZ^N-MLz gro^+t10.4 neur¹ neur^A101 neur^Scer\UAS.cPa neur^{ΔRING.Scer\UAS.T:Avic\GFP-EGFP} Scer\GAL4^bi-omb-Gal4 Scer\GAL4^hs.2sev Scer\GAL4^{neur-GAL4-A101} Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} T:Avic\GFP^EGFP
Constructs (5)
	P{GAL4-Act5C(FRT.CD2).P} P{GAL4-Hsp70.sev} P{UAS-Dl.L} P{UAS-neur.P} P{UAS-neur.ΔRING::EGFP}
Genes (11)
	ase ct Dl Ecol\lacZ Fas3 gro N neur Scer\GAL4 T:Avic\GFP wg
Insertions (7)
	P{GAL4}bi^omb-Gal4 P{GAL4-Act5C(FRT.CD2).P}S P{GAL4-Hsp70.sev}K25 P{GawB}neur^GAL4-A101 P{lacZ}N^MLz P{lArB}Dl^A326.2F3 P{lArB}neur^A101