Reference Report

Reference
Citation	St Pierre, S.E., Galindo, M.I., Couso, J.P., Thor, S. (2002). Control of Drosophila imaginal disc development by rotund and roughened eye: differentially expressed transcripts of the same gene encoding functionally distinct zinc finger proteins. Development 129(5): 1273--1281. (Export to RIS)
FlyBase ID	FBrf0144823
Publication Type	Research paper
PubMed ID	11874922
PubMed Abstract	The Drosophila rotund gene is required in the wings, antenna, haltere, proboscis and legs. A member of the Rac family of GTPases, denoted the rotund racGAP gene, was previously identified in the rotund region. However, previous studies indicated that rotund racGAP was not responsible for the rotund phenotypes and that the rotund gene had yet to be identified. We have isolated the rotund gene and show that it is a member of the Krüppel family of zinc finger genes. The adjacent roughened eye locus specifically affects the eye and is genetically separable from rotund. However, roughened eye and rotund are tightly linked, and we have therefore also isolated the roughened eye transcript. Intriguingly, we show that roughened eye is part of the rotund gene but is represented by a different transcript. The rotund and roughened eye transcripts result from the utilization of two different promoters that direct expression in non-overlapping domains in the larval imaginal discs. The predicted Rotund and Roughened Eye proteins share the same C-terminal region, including the zinc finger domain, but differ in their N-terminal regions. Each cDNA can rescue only the corresponding mutation and show negative effects when expressed in each others domain of expression. These results indicate that in addition to the differential expression of rotund and roughened eye, their proteins have distinct activities. rotund and roughened eye act downstream of early patterning genes such as dachshund and appear to be involved in Notch signaling by regulating Delta, scabrous and SERRATE:
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (2)
	Df(3R)rn19 Df(3R)rn20
Alleles (22)
	Ecol\lacZ^rn-89 Ecol\lacZ^Scer\UAS.cUa rn^1-5 rn^2-1 rn¹⁶ rn¹⁹ rn⁸⁹ rn^GAL4-5 rn^GAL4-13 rn^GAL4-14 rn^roe-3 rn^roe.Scer\UAS rn^{Scer\UAS.cSPa} rn^unspecified rn^Δ2-2 Scer\GAL4^GMR.PF Scer\GAL4^rn-GAL4-5 Scer\GAL4^rn-GAL4-13 Scer\GAL4^rn-GAL4-14 Scer\GAL4^sev.EP Ser¹ w^+mC
Constructs (5)
	P{GAL4-ninaE.GMR} P{sevEP-GAL4.B} P{UAS-lacZ.U} P{UAS-rn.SP} P{UAS-roe.SP}
Genes (13)
	bab1 boss dac Dl Ecol\lacZ elav RacGAP84C rn sca Scer\GAL4 Ser sqz w
Insertions (11)
	P{5'3'}rn^1-5 P{5'3'}rn^2-1 P{GawB}rn^GAL4-5 P{GawB}rn^GAL4-13 P{GawB}rn^GAL4-14 P{lArB}rn⁸⁹ P{lArB}rn^Δ2-2 P{UAS-rn.SP}1 P{UAS-rn.SP}32 P{UAS-roe.SP}18 P{UAS-roe.SP}88