Reference Report

Reference
Citation	Xin, S., Weng, L., Xu, J., Du, W. (2002). The role of RBF in developmentally regulated cell proliferation in the eye disc and in Cyclin D/Cdk4 induced cellular growth. Development 129(6): 1345--1356. (Export to RIS)
FlyBase ID	FBrf0144826
Publication Type	Research paper
PubMed ID	11880344
PubMed Abstract	During Drosophila eye development, cell proliferation is coordinated with differentiation. Immediately posterior to the morphogenetic furrow, cells enter a synchronous round of S phase called second mitotic wave. We have examined the role of RBF, the Drosophila RB family homolog, in cell cycle progression in the second mitotic wave. RBF-280, a mutant form of RBF that has four putative cdk phosphorylation sites mutated, can no longer be regulated by Cyclin D or Cyclin E. Expression of RBF-280 in the developing eye revealed that RBF-280 does not inhibit G1/S transition in the second mitotic wave, rather it delays the completion of S phase and leads to abnormal eye development. These observations suggest that RB/E2F control the rate of S-phase progression instead of G1/S transition in the second mitotic wave. Characterization of the role of RBF in Cyclin D/Cdk4-mediated cellular growth showed that RBF-280 blocks Cyclin D/Cdk4 induced cellular growth in the proliferating wing disc cells but not in the non-dividing eye disc cells. By contrast, RBF-280 does not block activated Ras-induced cellular growth. These results suggest that the ability of Cyclin D/Cdk4 to drive growth in the proliferating wing cells is distinct from that in the none-dividing eye cells or the ability of activated Ras to induce growth, and that RBF may have a role in regulating growth in the proliferating wing discs.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (38)
	cdc2c^cXa Cdk4^cXa Cdk4^Scer\UAS.cMa CycD^cXa CycD^GMR.PE CycD^Scer\UAS.cMa CycE^cXa dap^GMR.PdN dap^unspecified Dp^Act5C.PD E2f^Act5C.PD Ecol\CAT^E2f.4 Ras85D^V12.Scer\UAS Rbf^10.cXa Rbf^10.hs Rbf^10.Scer\UAS Rbf^16.Scer\UAS Rbf^30.cXa Rbf^30.hs Rbf^30.Scer\UAS Rbf^223.cXa Rbf^223.Scer\UAS Rbf^280.cXa Rbf^280.GMR Rbf^280.hs Rbf^280.Scer\UAS Rbf^555L.hs Rbf^{555L.Scer\UAS} Rbf^596W.hs Rbf^{596W.Scer\UAS} Rbf^Act5C.PD Rbf^GMR.PD Rbf^M4.cXa Rbf^Scer\UAS.cDa Rbf^unspecified Scer\GAL4^Act5C.PP Scer\GAL4^ey.PH Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}
Constructs (23)
	P{GAL4-Act5C(FRT.CD2).P} P{GAL4-Act5C(-FRT).P} P{GAL4-ey.H} P{GMR-CycD.E} P{GMR-dap} P{GMR-Rbf.280} P{GMR-Rbf} P{hs-Rbf.30} P{hs-Rbf.280} P{hs-Rbf.555L} P{hs-Rbf.596W} P{hs-Rbf.X} P{UAS-Cdk4.M} P{UAS-CycD.M} P{UAS-Ras85D.V12} P{UAS-Rbf.10} P{UAS-Rbf.16} P{UAS-Rbf.30} P{UAS-Rbf.223} P{UAS-Rbf.280} P{UAS-Rbf.555L} P{UAS-Rbf.596W} P{UAS-Rbf.D}
Genes (13)
	cdc2c Cdk4 CycD CycE dap Dp E2f Ecol\CAT His3 mus209 Ras85D Rbf Scer\GAL4