Reference Report

Reference
Citation	Punzo, C., Seimiya, M., Flister, S., Gehring, W.J., Plaza, S. (2002). Differential interactions of eyeless and twin of eyeless with the sine oculis enhancer. Development 129(3): 625--634. (Export to RIS)
FlyBase ID	FBrf0144839
Publication Type	Research paper
PubMed ID	11830564
PubMed Abstract	Drosophila eye development is under the control of early eye specifying genes including eyeless (ey), twin of eyeless (toy), eyes absent (eya), dachshund (dac) and sine oculis (so). They are all conserved between vertebrates and insects and they interact in a combinatorial and hierarchical network to regulate each other expression. so has been shown to be directly regulated by ey through an eye-specific enhancer (so10). We further studied the regulation of this element and found that both Drosophila Pax6 proteins namely EY and TOY bind and positively regulate so10 expression through different binding sites. By targeted mutagenesis experiments, we disrupted these EY and TOY binding sites and studied their functional involvement in the so10 enhancer expression in the eye progenitor cells. We show a differential requirement for the EY and TOY binding sites in activating so10 during the different stages of eye development. Additionally, in a rescue experiment performed in the so(1) mutant, we show that the EY and TOY binding sites are required for compound eye and ocellus development respectively. Altogether, these results suggest a differential requirement for EY and TOY to specify the development of the two types of adult visual systems, namely the compound eye and the ocellus.
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (31)
	Ecol\lacZ^Scer\UAS.cUa Ecol\lacZ^so.7 Ecol\lacZ^so.9 Ecol\lacZ^{so.10.EY.TOYmt.1.2.5} Ecol\lacZ^{so.10.EY+TOYmt.1-5} Ecol\lacZ^so.10.TOYmt Ecol\lacZ^so.10 ey² ey^J5.71 ey^Scer\UAS.cHa ey^{ΔHD.Scer\UAS} ey^{ΔPD.Scer\UAS} eya¹ Scer\GAL4^dpp.blk1 Scer\GAL4^ey.PH Scer\GAL4^{so.7.EY.TOYmt.1.2.5} Scer\GAL4^{so.7.EY+TOYmt.1-5} Scer\GAL4^so.7.TOYmt Scer\GAL4^so.7 Scer\GAL4^{so.10.EY.TOYmt.1.2.5} Scer\GAL4^{so.10.EY+TOYmt.1-5} Scer\GAL4^so.10.TOYmt Scer\GAL4^so.10 so¹ so^Scer\UAS.cPa sv^spa-pol toy^G7.39 toy^Scer\UAS.cCa toy^{ΔHD.Scer\UAS} toy^{ΔPD.Scer\UAS} w^+mC
Constructs (24)
	P{GAL4-dpp.blk1} P{GAL4-ey.H} P{so7-GAL4.EY/TOYmt.1.2.5} P{so7-GAL4.EY+TOYmt.1-5} P{so7-GAL4.TOYmt} P{so7-GAL4} P{so7-lacZ} P{so9-lacZ} P{so10-GAL4.EY/TOYmt.1.2.5} P{so10-GAL4.EY+TOYmt.1-5} P{so10-GAL4.TOYmt} P{so10-GAL4} P{so-lacZ.10.EY/TOYmt.1.2.5} P{so-lacZ.10.EY+TOYmt.1-5} P{so-lacZ.10.TOYmt} P{so-lacZ.10} P{UAS-ey.H} P{UAS-ey.ΔHD} P{UAS-ey.ΔPD} P{UAS-lacZ.U} P{UAS-so.P} P{UAS-toy.C} P{UAS-toy.ΔHD} P{UAS-toy.ΔPD}
Genes (8)
	Ecol\lacZ ey eya Scer\GAL4 so sv toy w
Sequence features (6)
	so_so7 so-protein_bind-1 so-protein_bind-2 so-protein_bind-3 so-protein_bind-4 so-protein_bind-5