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Reference Report
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| Citation | Kalmykova, A.I., Shevelyov, Y.Y., Polesskaya, O.O., Dobritsa, A.A., Evstafieva, A.G., Boldyreff, B., Issinger, O.G., Gvozdev, V.A. (2002). CK2betates gene encodes a testis-specific isoform of the regulatory subunit of casein kinase 2 in Drosophila melanogaster. Europ. J. Biochem. 269(5): 1418--1427. (Export to RIS) | ||
| FlyBase ID | FBrf0144858 | ||
| Publication Type | Research paper | ||
| PubMed ID | 11874456 | ||
| PubMed Abstract | An earlier described CK2(beta)tes gene of Drosophila melanogaster is shown to encode a male germline specific isoform of regulatory beta subunit of casein kinase 2. Western-analysis using anti-CK2(beta)tes Ig revealed CK2(beta)tes protein in Drosophila testes extract. Expression of a CK2(beta)tes-beta-galactosidase fusion protein driven by the CK2(beta)tes promoter was found in transgenic flies at postmitotic stages of spermatogenesis. Examination of biochemical characteristics of a recombinant CK2(beta)tes protein expressed in Escherichia coli revealed properties similar to those of CK2beta: (a) CK2(beta)tes protein stimulates CK2alpha catalytic activity toward synthetic peptide; (b) it inhibits phosphorylation of calmodulin and mediates stimulation of CK2alpha by polylysine; (c) it is able to form (CK2(beta)tes)2 dimers, as well as (CK2alpha)2(CK2(beta)tes)2 tetramers. Using the yeast two-hybrid system and coimmunoprecipitation analysis of protein extract from Drosophila testes, we demonstrated an association between CK2(beta)tes and CK2alpha. Northern-analysis has shown that another regulatory (beta') subunit found recently in D. melanogaster genome is also testis-specific. Thus, we describe the first example of two tissue-specific regulatory subunits of CK2 which might serve to provide CK2 substrate recognition during spermatogenesis. | ||
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| Language of Publication | English | ||
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| Publication Type | Journal | ||
| Abbreviation | Europ. J. Biochem. | ||
| Title | European Journal of Biochemistry | ||
| Publication Year | 1967-2004 | ||
| ISBN/ISSN | 0014-2956 | ||
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Data from Reference
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| Alleles (4)
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| Constructs (3)
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| Genes (6)
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