Reference Report

Reference
Citation	Tsruya, R., Schlesinger, A., Reich, A., Gabay, L., Sapir, A., Shilo, B.Z. (2002). Intracellular trafficking by Star regulates cleavage of the Drosophila EGF receptor ligand Spitz. Genes Dev. 16(2): 222--234. (Export to RIS)
FlyBase ID	FBrf0144878
Publication Type	Research paper
PubMed ID	11799065
PubMed Abstract	Spitz (Spi) is a TGFalpha homolog that is a cardinal ligand for the Drosophila EGF receptor throughout development. Cleavage of the ubiquitously expressed transmembrane form of Spi (mSpi) precedes EGF receptor activation. We show that the Star and Rhomboid (Rho) proteins are necessary for Spi cleavage in Drosophila cells. Complexes between the Spi and Star proteins, as well as between the Star and Rho proteins were identified, but no Spi-Star-Rho triple complex was detected. This observation suggests a sequential activity of Star and Rho in mSpi processing. The interactions between Spi and Star regulate the intracellular trafficking of Spi. The Spi precursor is retained in the periphery of the nucleus. Coexpression of Star promotes translocation of Spi to a compartment where Rho is present both in cells and in embryos. A Star deletion construct that maintains binding to Spi and Rho, but is unable to facilitate Spi translocation, lost biological activity. These results underscore the importance of regulated intracellular trafficking in processing of a TGFalpha family ligand.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Alleles (21)
	Bmor\S^{Scer\UAS.T:Ivir\HA1} rho^{Scer\UAS.T:Zzzz\FLAG} rho^{Scer\UAS.T:Zzzz\TAP} rho^{Scer\UAS.T.T:Ivir\HA1} S⁵⁴ S^Scer\UAS.cGa S^{Scer\UAS.NTM.T:Ivir\HA1} S^{Scer\UAS.T:Ivir\HA1} S^{Scer\UAS.TMC.T:Ivir\HA1} Scer\GAL4^Bx-MS1096 Scer\GAL4^GMR.PF Scer\GAL4^prd.RG1 spi^{s.Scer\UAS.T:Avic\GFP-EGFP} spi^Scer\UAS.cSa spi^{Scer\UAS.T:Avic\GFP} spi^{Scer\UAS.T:Zzzz\FLAG} spi^{Scer\UAS.T:Zzzz\TAP} spi^{Scer\UAS.Δ16} spi^{Scer\UAS.ΔEGF.s.T:Avic\GFP} spi^{Scer\UAS.ΔEGF.T:Avic\GFP} spi^{Scer\UAS.ΔIC.T:Avic\GFP}
Constructs (19)
	P{GAL4-ninaE.GMR} P{GAL4-prd.F} P{UAS-BmS.HA1} P{UAS-rho.FLAG} P{UAS-rho.T.HA} P{UAS-rho.TAP} P{UAS-spi.FLAG} P{UAS-spi.m.GFP} P{UAS-spi.m} P{UAS-spi.s.GFP} P{UAS-spi.s.T:GFP} P{UAS-spi.T:GFP} P{UAS-spi.TAP} P{UAS-spi.Δ16} P{UAS-spi.ΔIC.T:GFP} P{UAS-Star.HA1} P{UAS-Star.NTM.HA1} P{UAS-Star.TMC.HA1} P{UAS-Star}
Genes (9)
	Bmor\S rho S Scer\GAL4 spi T:Avic\GFP T:Ivir\HA1 T:Zzzz\FLAG T:Zzzz\TAP
Insertions (1)
	P{GawB}Bx^MS1096