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Citation
Roy, F., Laberge, G., Douziech, M., Ferland-McCollough, D., Therrien, M. (2002). KSR is a scaffold required for activation of the ERK/MAPK module.  Genes Dev. 16(4): 427--438.
FlyBase ID
FBrf0144882
Publication Type
Research paper
Abstract

Mechanisms that regulate signal propagation through the ERK/MAPK pathway are still poorly understood. Several proteins are suspected to play critical roles in this process. One of these is Kinase Suppressor of Ras (KSR), a component previously identified in RAS-dependent genetic screens in Drosophila and Caenorhabditis elegans. Here, we show that KSR functions upstream of MEK within the ERK/MAPK module. In agreement with this, we found that KSR facilitates the phosphorylation of MEK by RAF. We further show that KSR associates independently with RAF and MEK, and that these interactions lead to the formation of a RAF/MEK complex, thereby positioning RAF in close proximity to its substrate MEK. These findings suggest that KSR functions as a scaffold that assembles the RAF/MEK functional pair.

PubMed ID
PubMed Central ID
PMC155344 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genes Dev.
    Title
    Genes & Development
    Publication Year
    1987-
    ISBN/ISSN
    0890-9369
    Data From Reference
    Alleles (2)
    Gene Groups (1)
    Genes (8)
    Transgenic Constructs (2)