Reference Report

Reference
Citation	Csink, A.K., Bounoutas, A., Griffith, M.L., Sabl, J.F., Sage, B.T. (2002). Differential gene silencing by trans-heterochromatin in Drosophila melanogaster. Genetics 160(1): 257--269. (Export to RIS)
FlyBase ID	FBrf0144893
Publication Type	Research paper
PubMed ID	11805061
PubMed Abstract	The brown(Dominant) (bw(D)) allele contains a large insertion of heterochromatin leading to the trans-inactivation of the wild-type allele in bw(D)/bw(+) heterozygous flies. This silencing is correlated with the localization of bw(+) to a region of the interphase nucleus containing centric heterochromatin. We have used a series of transgene constructs inserted in the vicinity of the bw locus to demarcate both the extent of bw(D) influence along the chromosome and the relative sensitivities of various genes. Examples of regulatory regions that are highly sensitive, moderately sensitive, and insensitive were found. Additionally, by using the same transgene at increasing distances from the bw(D) insertion site in trans we were able to determine the range of influence of the heterochromatic neighborhood in terms of chromosomal distance. When the transgene was farther away from bw, there was, indeed, a tendency for it to be less trans-inactivated. However, insertion site also influenced silencing: a gene 86 kb away was trans-inactivated, while the same transgene 45 kb away was not. Thus location, distance, and gene-specific differences all influence susceptibility to trans-silencing near a heterochromatic neighborhood. These results have important implications for the ability of nuclear positioning to influence the expression of large blocks of a chromosome.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (2)
	Df(2R)M41A10 Dp(?;2)bw^D
Alleles (17)
	apt^k11531 bw^D Dcp-1⁰¹⁸⁶² Dcp-1⁰⁸⁸⁵⁹ Dcp-1^k05606 Dcp-1^mgb48 Ecol\lacZ^apt-03041 Ecol\lacZ^Dcp-1-01862 Ecol\lacZ^Dcp-1-08859 Ecol\lacZ^Dcp-1-k05606 Ecol\lacZ^Nup214-10444 Ecol\lacZ^RabX1-k06908 Ecol\lacZ^s4830 Hsp26^T:Hvug\SIP1 RabX1^ab28 w^+mC w^+mW.hs
Constructs (2)
	P{hsp26-pt-T} P{lacW}
Genes (8)
	apt bw Dcp-1 Ecol\lacZ Hsp26 RabX1 T:Hvug\SIP1 w
Insertions (15)
	P{hsp26-pt-T}ab8 P{hsp26-pt-T}Dcp-1^mgb48 P{hsp26-pt-T}RabX1^ab28 P{lacW}apt^k11531 P{lacW}apt^k15608 P{lacW}Dcp-1^k05606 P{lacW}l(2)k07136^k07136 P{lacW}l(2)k09913^k09913 P{lacW}l(2)k09931^k09931 P{lacW}l(2)s4830^s4830 P{lacW}mi^k06908 P{PZ}apt⁰³⁰⁴¹ P{PZ}Dcp-1⁰¹⁸⁶² P{PZ}Dcp-1⁰⁸⁸⁵⁹ P{PZ}Nup214¹⁰⁴⁴⁴