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Reference
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| Citation |
Lozovsky, E.R., Nurminsky, D., Wimmer, E.A., Hartl, D.L. (2002). Unexpected Stability of mariner Transgenes in Drosophila. Genetics 160(2): 527--535. |
| FlyBase ID |
FBrf0144896 |
| Type of publication |
Research paper |
| Offprint Available |
No |
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External Crossreferences
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| PubMed ID |
11861559 |
| PubMed Abstract |
A number of mariner transformation vectors based on the mauritiana subfamily of transposable elements were introduced into
the genome of Drosophila melanogaster and examined for their ability to be mobilized by the mariner transposase. Simple insertion
vectors were constructed from single mariner elements into which exogenous DNA ranging in size from 1.3 to 4.5 kb had been
inserted; composite vectors were constructed with partial or complete duplications of mariner flanking the exogenous DNA.
All of the simple insertion vectors showed levels of somatic and germline excision that were at least 100-fold lower than
the baseline level of uninterrupted mariner elements. Although composite vectors with inverted duplications were unable to
be mobilized at detectable frequencies, vectors with large direct duplications of mariner could be mobilized. A vector consisting
of two virtually complete elements flanking exogenous DNA yielded a frequency of somatic eye-color mosaicism of approximately
10% and a frequency of germline excision of 0.04%. These values are far smaller than those observed for uninterrupted elements.
The results imply that efficient mobilization of mariner in vivo requires the presence and proper spacing of sequences internal
to the element as well as the inverted repeats.
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| Biosis |
2002.217427 |
| Zoological record |
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Associated Information
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| Comments |
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| Text of personal communication |
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Related Publications
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Also Published As
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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Published In
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| Abbreviation |
Genetics |
| Title |
Genetics |
| Authors |
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| Volume range |
1- |
| Year range |
1916- |
| Page range |
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| Publisher |
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| Place of publication |
Austin, TX, etc |
| Language of publication |
English |
| ISBN/ISSN |
0016-6731 |
| CODEN |
GENTAE |
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Data from Reference
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| Alleles (10)
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| Constructs (18)
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| Genes (4)
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