Reference Report

Reference
Citation	Stempfl, T., Vogel, M., Szabo, G., Wulbeck, C., Liu, J., Hall, J.C., Stanewsky, R. (2002). Identification of circadian-clock-regulated enhancers and genes of Drosophila melanogaster by transposon mobilization and luciferase reporting of cyclical gene expression. Genetics 160(2): 571--593. (Export to RIS)
FlyBase ID	FBrf0144900
Publication Type	Research paper
PubMed ID	11861563
PubMed Abstract	A new way was developed to isolate rhythmically expressed genes in Drosophila by modifying the classic enhancer-trap method. We constructed a P element containing sequences that encode firefly luciferase as a reporter for oscillating gene expression in live flies. After generation of 1176 autosomal insertion lines, bioluminescence screening revealed rhythmic reporter-gene activity in 6% of these strains. Rhythmically fluctuating reporter levels were shown to be altered by clock mutations in genes that specify various circadian transcription factors or repressors. Intriguingly, rhythmic luminescence in certain lines was affected by only a subset of the pacemaker mutations. By isolating genes near 13 of the transposon insertions and determining their temporal mRNA expression pattern, we found that four of the loci adjacent to the trapped enhancers are rhythmically expressed. Therefore, this approach is suitable for identifying genetic loci regulated by the circadian clock. One transposon insert caused a mutation in the rhythmically expressed gene numb. This novel numb allele, as well as previously described ones, was shown to affect the fly's rhythm of locomotor activity. In addition to its known role in cell fate determination, this gene and the phosphotyrosine-binding protein it encodes are likely to function in the circadian system.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Alleles (48)
	awd^III129 CG2201^1-45 CG6145^90-3 CG6145^rev1 CG6145^rev2 CG13928^6-63 Clk^Jrk cpx^3-50 cyc⁰¹ hts^4-29 lola^6-4 numb¹ numb² numb³ numb^nuts numb^SW osa^9-24 per⁰¹ per^T Ppyr\LUC^2-49 Ppyr\LUC^3-70 Ppyr\LUC^3-80 Ppyr\LUC^3-89 Ppyr\LUC^4-16 Ppyr\LUC^5-1 Ppyr\LUC^6-48 Ppyr\LUC^awd-III129 Ppyr\LUC^CG2201-1-45 Ppyr\LUC^CG6145-90-3 Ppyr\LUC^CG13928-6-63 Ppyr\LUC^{complexin-3-50} Ppyr\LUC^hts-4-29 Ppyr\LUC^lola-6-4 Ppyr\LUC^numb-nuts Ppyr\LUC^osa-9-24 Ppyr\LUC^P\T.PS Ppyr\LUC^per.PB Ppyr\LUC^qsm-1-17 Ppyr\LUC^SNF4Aγ-8-35 Ppyr\LUC^SpdS-1 Ppyr\LUC^tws-90-14 qsm^1-17 SNF4Aγ^8-35 SpdS¹ tim⁰¹ tim⁰³ tws^90-14 w^+mC
Constructs (3)
	P{luc-sniffer} P{per-luc} pP{luc-sniffer}
Genes (53)
	awd CalpA cer CG1896 CG2003 CG2053 CG2201 CG3769 CG4467 CG5862 CG6051 CG6145 CG6241 CG9766 CG9780 CG10809 CG12268 CG13928 CG31249 CG31251 CG33156 Clk cnc cpx Crc cyc Df31 GC Gdh hts knk lola Men-b Mgat1 nero Nnf1a n-syb numb osa per Ppyr\LUC psq Pxt qsm RasGAP1 Scm SNF4Aγ Snmp1 SpdS tim tws Vmat w
Insertions (21)
	P{luc-sniffer}2-49 P{luc-sniffer}3-70 P{luc-sniffer}3-80 P{luc-sniffer}3-89 P{luc-sniffer}4-16 P{luc-sniffer}5-1 P{luc-sniffer}6-48 P{luc-sniffer}awd^III129 P{luc-sniffer}CG2201^1-45 P{luc-sniffer}CG6145^90-3 P{luc-sniffer}CG13928^6-63 P{luc-sniffer}cpx^3-50 P{luc-sniffer}hts^4-29 P{luc-sniffer}lola^6-4 P{luc-sniffer}numb^nuts P{luc-sniffer}osa^9-24 P{luc-sniffer}qsm^1-17 P{luc-sniffer}SNF4Aγ^8-35 P{luc-sniffer}SpdS¹ P{luc-sniffer}tws^90-14 P{luc-sniffer}X-90
Natural transposons (1)
	Rt1b