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Citation
Hudson, A.M., Cooley, L. (2002). A subset of dynamic actin rearrangements in Drosophila requires the Arp2/3 complex.  J. Cell Biol. 156(4): 677--687.
FlyBase ID
FBrf0144947
Publication Type
Research paper
Abstract

The Arp2/3 complex has been shown to dramatically increase the slow spontaneous rate of actin filament nucleation in vitro, and it is known to be important for remodeling the actin cytoskeleton in vivo. We isolated and characterized loss of function mutations in genes encoding two subunits of the Drosophila Arp2/3 complex: Arpc1, which encodes the homologue of the p40 subunit, and Arp3, encoding one of the two actin-related proteins. We used these mutations to study how the Arp2/3 complex contributes to well-characterized actin structures in the ovary and the pupal epithelium. We found that the Arp2/3 complex is required for ring canal expansion during oogenesis but not for the formation of parallel actin bundles in nurse cell cytoplasm and bristle shaft cells. The requirement for Arp2/3 in ring canals indicates that the polymerization of actin filaments at the ring canal plasma membrane is important for driving ring canal growth.

PubMed ID
PubMed Central ID
PMC2174088 (PMC) (EuropePMC)
Related Publication(s)
Note

Extending the Arp2/3 complex and its regulation beyond the leading edge.
Miller, 2002, J. Cell Biol. 156(4): 591--593 [FBrf0144946]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Cell Biol.
    Title
    Journal of Cell Biology
    Publication Year
    1966-
    ISBN/ISSN
    0021-9525
    Data From Reference
    Aberrations (1)
    Alleles (13)
    Gene Groups (1)
    Genes (10)
    Insertions (1)
    Experimental Tools (2)
    Transgenic Constructs (5)