Reference Report

Reference
Citation	Shyamala, B.V., Bhat, K.M. (2002). A positive role for Patched-Smoothened signaling in promoting cell proliferation during normal head development in Drosophila. Development 129(8): 1839--1847. (Export to RIS)
FlyBase ID	FBrf0146982
Publication Type	Research paper
External Crossreferences
PubMed ID	11934850
PubMed Abstract	The transmembrane receptor Patched regulates several developmental processes in both invertebrates and vertebrates. In vertebrates, Patched also acts as a tumor suppressor. The Patched pathway normally operates by negatively regulating Smoothened, a G-protein-coupled receptor; binding of Hedgehog ligand to Patched relieves this negative interaction and allows signaling by Smoothened. We show that Ptc regulates Drosophila head development by promoting cell proliferation in the eye-antennal disc. During head morphogenesis, Patched positively interacts with Smoothened, which leads to the activation of Activin type I receptor Baboon and stimulation of cell proliferation in the eye-antennal disc. Thus, loss of Ptc or Smoothened activity affects cell proliferation in the eye-antennal disc and results in adult head capsule defects. Similarly, reducing the dose of smoothened in a patched background enhances the head defects. Consistent with these results, gain-of-function Hedgehog interferes with the activation of Baboon by Patched and Smoothened, leading to a similar head capsule defect. Expression of an activated form of Baboon in the patched domain in a patched mutant background completely rescues the head defects. These results provide insight into head morphogenesis, a process we know very little about, and reveal an unexpected non-canonical positive signaling pathway in which Patched and Smoothened function to promote cell proliferation as opposed to repressing it.
BIOSIS ID	2002.497373
Zoological Record ID
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Text of Personal Communication
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Secondary IDs
Language of Publication	English
Additional Languages of Abstract
ISBN
Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Editors
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (2)
	Df(2R)Np3 Df(3L)H99
Alleles (25)
	babo³² babo^k07737 babo^{Q302D.Scer\UAS} hh^hs.PI hh^ts2 ptc⁴ ptc⁵ ptc⁷ ptc⁸ ptc⁹ ptc¹⁰ ptc¹² ptc¹⁴ ptc¹⁷ ptc^559.1 ptc^H84 ptc^hdl ptc^IIC99 ptc^S2 put¹⁰⁴⁶⁰ Scer\GAL4^69B Scer\GAL4^ptc-559.1 smo¹ smo^D16 smo^unspecified
Constructs (2)
	P{HShh.I} P{UAS-babo.Q302D}
Genes (9)
	babo grim hh ptc put rpr Scer\GAL4 smo W
Insertions (2)
	P{GawB}69B P{GawB}ptc^559.1
Polypeptides (2)
	hh-XP ptc-XP