Reference Report

Reference
Citation	Melnikova, L., Gause, M., Georgiev, P. (2002). The gypsy insulators flanking yellow enhancers do not form a separate transcriptional domain in Drosophila melanogaster. The enhancers can activate an isolated yellow promoter. Genetics 160(4): 1549--1560. (Export to RIS)
FlyBase ID	FBrf0147065
Publication Type	Research paper
PubMed ID	11973309
PubMed Abstract	The best-characterized insulator in Drosophila melanogaster is the Su(Hw)-binding region contained within the gypsy retrotransposon. In the y(2) mutant, Su(Hw) protein partially inhibits yellow transcription by blocking the function of transcriptional enhancers located distally from the yellow promoter with respect to gypsy. Previously we have shown that yellow enhancers can overcome inhibition by a downstream insulator in the y(rh1) allele, when a second gypsy element is located upstream of the enhancers. To understand how two insulators neutralize each other, we isolated various deletions that terminate in the regulatory region of the y(rh1) allele. To generate these alleles we used DNA elongation by gene conversion of the truncated chromosomes at the end of the yellow regulatory region. We found that gypsy insulator can function at the end of the truncated chromosome. Addition of the gypsy insulator upstream of the yellow enhancers overcomes the enhancer-blocking activity of the gypsy insulator inserted between the yellow enhancers and promoter. These results suggest that the gypsy insulators do not form separate transcriptional domains that delimit the interactions between enhancers and promoters.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (11)
	Df(1)y^TD2h1 Df(1)y^TD2h2 Df(1)y^TD2h3 Df(1)y^TD2h4 Df(1)y^TDh11 Df(1)y^TDlh1 Df(1)y^TDlh2 Df(1)y^TDmh1 Df(1)y^TDrh1 Df(1)y^TDrh2 Df(1)yacw
Alleles (18)
	En(tc)^unspecified su(Hw)¹ su(Hw)² su(Hw)^V y² y^lh11 y^mh32 y^rh1 y^TD2h1 y^TD2h2 y^TD2h3 y^TD2h4 y^TDh11 y^TDlh1 y^TDlh2 y^TDmh1 y^TDrh1 y^TDrh2
Genes (5)
	ac En(tc) gypsy\su(Hw)BR su(Hw) y
Insertions (11)
	gypsy{}y² gypsy{}y^lh11b gypsy{}y^lh11c gypsy{}y^mh32a gypsy{}y^mh32c gypsy{}y^rh1a H{}y^dh1a H{}y^dh1b H{}y^lh11a H{}y^mh32b H{}y^rh1b