Reference Report

Reference
Citation	Harden, N., Ricos, M., Yee, K., Sanny, J., Langmann, C., Yu, H., Chia, W., Lim, L. (2002). Drac1 and Crumbs participate in amnioserosa morphogenesis during dorsal closure in Drosophila. J. Cell Sci. 115(10): 2119--2129. (Export to RIS)
FlyBase ID	FBrf0147105
Publication Type	Research paper
PubMed ID	11973353
PubMed Abstract	Dorsal closure of the Drosophila embryo involves morphological changes in two epithelia, the epidermis and the amnioserosa, and is a popular system for studying the regulation of epithelial morphogenesis. We previously implicated the small GTPase Drac1 in the assembly of an actomyosin contractile apparatus, contributing to cell shape change in the epidermis during dorsal closure. We now present evidence that Drac1 and Crumbs, a determinant of epithelial polarity, are involved in setting up an actomyosin contractile apparatus that drives amnioserosa morphogenesis by inducing apical cell constriction. Expression of constitutively active Drac1 causes excessive constriction of amnioserosa cells and contraction of the tissue, whereas expression of dominant-negative Drac1 impairs amnioserosa morphogenesis. These Drac1 transgenes may be acting through their effects on the amnioserosa cytoskeleton, as constitutively active Drac1 causes increased staining for F-actin and myosin, whereas dominant-negative Drac1 reduces F-actin levels. Overexpression of Crumbs causes premature cell constriction in the amnioserosa, and dorsal closure defects are seen in embryos homozygous for hypomorphic crumbs alleles. The ability of constitutively active Drac1 to cause contraction of the amnioserosa is impaired in a crumbs mutant background. We propose that amnioserosa morphogenesis is a useful system for studying the regulation of epithelial morphogenesis by Drac1.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Sci.
Title	Journal of Cell Science
Publication Year	1966-
ISBN/ISSN	0021-9533
Data from Reference
Aberrations (1)
	Df(3R)crb87-5
Alleles (20)
	Cdc42^N17.Scer\UAS Cdc42^V12.Scer\UAS crb^11A22 crb^S010409 crb^S025807b crb^S025817 crb^S025819 crb^S050920 crb^Scer\UAS.cWa Ecol\lacZ^Scer\UAS.cBa Hsap\RHOA^{N19.Scer\UAS.T:Ivir\HA1} Hsap\RHOA^{V14.Scer\UAS.T:Ivir\HA1} Rac1^N17.Scer\UAS Rac1^V12.Scer\UAS Ras85D^N17.Scer\UAS Ras85D^Q13.Scer\UAS Rho1^N19.Scer\UAS Scer\GAL4^332.3 Scer\GAL4^c381 Scer\GAL4^hs.PB
Constructs (9)
	P{GAL4-Hsp70.PB} P{UAS-Cdc42.N17} P{UAS-Cdc42.V12} P{UAS-crb.wt} P{UAS-lacZ.B} P{UAS-Rac1.N17} P{UAS-Rac1.V12} P{UAS-Ras85D.Q13} P{UAS-Rho1.N19}
Genes (12)
	Act5C Act42A Cdc42 crb Ecol\lacZ Hsap\RHOA Rac1 Ras85D Rho1 Scer\GAL4 T:Ivir\HA1 zip
Insertions (7)
	P{GawB}332.3 P{GawB}c381 P{lacW}crb^S010409 P{lacW}crb^S025807b P{lacW}crb^S025817 P{lacW}crb^S025819 P{lacW}crb^S050920