Reference Report

Reference
Citation	Folberg-Blum, A., Sapir, A., Shilo, B.Z., Oren, M. (2002). Overexpression of mouse Mdm2 induces developmental phenotypes in Drosophila. Oncogene 21(15): 2413--2417. (Export to RIS)
FlyBase ID	FBrf0147178
Publication Type	Research paper
PubMed ID	11948425
PubMed Abstract	The Mdm2 proto-oncogene is amplified and over-expressed in a variety of tumors. One of the major functions of Mdm2 described to date is its ability to modulate the levels and activity of the tumor suppressor protein p53. Mdm2 binds to the N-terminus of p53 and, through its action as an E3 ubiquitin ligase, targets p53 for rapid proteasomal degradation. Mdm2 can also bind to other cellular proteins such as hNumb, E2F1, Rb and Akt; however, the biological significance of these interactions is less clear. To gain insight into the function of Mdm2 in vivo, we have generated a transgenic Drosophila strain bearing the mouse Mdm2 gene. Ectopic expression of Mdm2, using the UAS/GAL4 system, causes eye and wing phenotypes in the fly. Analysis of wing imaginal discs from third instar larvae showed that expression of Mdm2 induces apoptosis. Crosses did not reveal genetic interactions between Mdm2 and the Drosophila homolog of E2F, Numb and Akt. These transgenic flies may provide a unique experimental model for exploring the molecular interactions of Mdm2 in a developmental context.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Oncogene
Title	Oncogene
Publication Year	1987-
ISBN/ISSN	0950-9232
Data from Reference
Alleles (8)
	Akt1⁰⁴²²⁶ BacA\p35^Scer\UAS.cHa E2f⁰⁷¹⁷² E2f^GMR.PD Mmus\Mdm2^Scer\UAS.cFa Scer\GAL4^Bx-MS1096 Scer\GAL4^en-e16E Scer\GAL4^GMR.PF
Constructs (4)
	P{GAL4-ninaE.GMR} P{GMRdE2F} P{UAS-Mdm2.F} P{UAS-p35.H}
Genes (7)
	Akt1 BacA\p35 E2f Mmus\Mdm2 numb p53 Scer\GAL4
Insertions (2)
	P{en2.4-GAL4}e16E P{GawB}Bx^MS1096
Natural transposons (1)
	P-element