Reference Report

Reference
Citation	Tree, D.R., Shulman, J.M., Rousset, R., Scott, M.P., Gubb, D., Axelrod, J.D. (2002). Prickle mediates feedback amplification to generate asymmetric planar cell polarity signaling. Cell 109(3): 371--381. (Export to RIS)
FlyBase ID	FBrf0148922
Publication Type	Research paper
PubMed ID	12015986
PubMed Abstract	Planar cell polarity signaling in Drosophila requires the receptor Frizzled and the cytoplasmic proteins Dishevelled and Prickle. From initial, symmetric subcellular distributions in pupal wing cells, Frizzled and Dishevelled become highly enriched at the distal portion of the cell cortex. We describe a Prickle-dependent intercellular feedback loop that generates asymmetric Frizzled and Dishevelled localization. In the absence of Prickle, Frizzled and Dishevelled remain symmetrically distributed. Prickle localizes to the proximal side of pupal wing cells and binds the Dishevelled DEP domain, inhibiting Dishevelled membrane localization and antagonizing Frizzled accumulation. This activity is linked to Frizzled activity on the adjacent cell surface. Prickle therefore functions in a feedback loop that amplifies differences between Frizzled levels on adjacent cell surfaces.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Cell
Title	Cell
Publication Year	1974-
ISBN/ISSN	0092-8674
Data from Reference
Alleles (15)
	dsh^{1.T:Avic\GFP-EGFP} dsh^hs.PA fz²³ fz^{arm.T:Avic\GFP-EGFP} fz^Scer\UAS.cAa pk^pk-sple-13 pk^Scer\UAS.cGa pk^{sple.Scer\UAS} Scer\GAL4^dpp.blk1 Scer\GAL4^en-e16E Scer\GAL4^ptc-559.1 Scer\GAL4^Scer\FRT.Ubx Scer\GAL4^Ubx.PdC stan^Scer\UAS.cUa T:Avic\GFP^EGFP
Constructs (10)
	P{arm-fz.GFP} P{dsh¹.EGFP} P{GAL4-dpp.blk1} P{GAL4-lacZ-Ubx(FRT.f⁺).dC} P{GAL4-lacZ-Ubx(-FRT).dC} P{Hs:dsh} P{UAS-fmi.U} P{UAS-fz.A} P{UAS-pk.G} P{UAS-sple⁺}
Genes (7)
	dsh Ecol\lacZ fz pk Scer\GAL4 stan T:Avic\GFP
Insertions (2)
	P{en2.4-GAL4}e16E P{GawB}ptc^559.1