Reference Report

Reference
Citation	Strutt, D., Johnson, R., Cooper, K., Bray, S. (2002). Asymmetric localization of frizzled and the determination of notch-dependent cell fate in the Drosophila eye. Curr. Biol. 12(10): 813--824. (Export to RIS)
FlyBase ID	FBrf0148932
Publication Type	Research paper
PubMed ID	12015117
PubMed Abstract	During patterning of the Drosophila eye, a critical step is the Notch-mediated cell fate decision that determines the identities of the R3/R4 photoreceptor pair in each ommatidium. Depending on the decision taken, the ommatidium adopts either the dorsal or ventral chiral form. This decision is directed by the activity of the planar polarity genes, and, in particular, higher activity of the receptor Frizzled confers R3 fate.We present evidence that Frizzled does not modulate Notch activity via Rho GTPases and a JNK cascade as previously proposed. We find that the planar polarity proteins Frizzled, Dishevelled, Flamingo, and Strabismus adopt asymmetric protein localizations in the developing photoreceptors. These protein localizations correlate with the bias of Notch activity between R3/R4, suggesting that they are necessary to modulate Notch activity between these cells. Additional data support a mechanism for regulation of Notch activity that could involve direct interactions between Dishevelled and Notch at the cell cortex.In the light of our findings, we conclude that Rho GTPases/JNK cascades are not major effectors of planar polarity in the Drosophila eye. We propose a new model for the control of R3/R4 photoreceptor fate by Frizzled, whereby asymmetric protein localization is likely to be a critical step in modulation of Notch activity. This modulation may occur via direct interactions between Notch and Dishevelled.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Aberrations (1)
	Df(2R)w45-30n
Alleles (26)
	bsk^flp147E Dl^Scer\UAS.cDa dsh¹ dsh³ Ecol\lacZ^HLHmδ.0.5 fz¹⁹ fz²⁰ fz²⁵ fz^{Act5C.T:Avic\GFP-EGFP} Jra^76-19 N^60g11 N^Mcd8 pk^pk-sple-13 pk^sple-1 Rac1^N17.Scer\UAS Rho1^72M1 Rho1^N19.sev Rho1^V14.sev Scer\GAL4^hs.2sev stan^E45 stan^E59 T:Avic\GFP^EGFP T:Avic\GFP^YFP Vang^A3 Vang^{Act5C.T:Avic\GFP-YFP} Vang^stbm-6
Constructs (8)
	P{Act5C(-FRT)fz.EGFP} P{Act5C-Vang.YFP} P{GAL4-Hsp70.sev} P{HLHmδ-lacZ.0.5} P{sE-Rho1.N19} P{sE-Rho1.V14} P{UAS-Dl.D} P{UAS-Rac1.N17}
Genes (17)
	bsk cora Dl dsh E(spl)mδ-HLH Ecol\lacZ elav fz Jra N pk Rac1 Rho1 Scer\GAL4 stan T:Avic\GFP Vang