Reference Report

Reference
Citation	Giraldez, A.J., Copley, R.R., Cohen, S.M. (2002). HSPG modification by the secreted enzyme Notum shapes the Wingless morphogen gradient. Dev. Cell 2(5): 667--676. (Export to RIS)
FlyBase ID	FBrf0148946
Publication Type	Research paper
PubMed ID	12015973
PubMed Abstract	The secreted signaling protein Wingless acts as a morphogen to pattern the imaginal discs of Drosophila. Here we report identification of a secreted repressor of Wingless activity, which we call Notum. Loss of Notum function leads to increased Wingless activity by altering the shape of the Wingless protein gradient. When overexpressed, Notum blocks Wingless activity. Notum encodes a member of the alpha/beta-hydrolase superfamily, with similarity to pectin acetylesterases. We present evidence that Notum influences Wingless protein distribution by modifying the heparan sulfate proteoglycans Dally-like and Dally. High levels of Wingless signaling induce Notum expression. Thus, Wingless contributes to shaping its own gradient by regulating expression of a protein that modifies its interaction with cell surface proteoglycans.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Alleles (29)
	arm^{S10.Scer\UAS.T:Hsap\MYC} dally^{MtnA.T:Ivir\HA1} dally^Scer\UAS.cJa dlp^{MtnA.T:Ivir\HA1} dlp^{Scer\UAS.T:Ivir\HA1} Notum¹ Notum^2.MtnA Notum² Notum³ Notum^72Da-1 Notum^{CG13076.Scer\UAS} Notum^EPg31259 Notum^{GT.MtnA.T:Hsap\GALNT3} Notum^{GT.Scer\UAS.T:Hsap\GALNT3} Notum^I6 Notum^MtnA.PG Notum^S237A.MtnA Notum^Scer\UAS.cGa Notum^unspecified Scer\GAL4^ap-md544 Scer\GAL4^dpp.blk1 Scer\GAL4^salm-459.2 Scer\GAL4^sd-SG29.1 Scer\GAL4^wg-Gal4 Scer\GAL4^αTub84B.PL sfl^cGa sgg^Scer\UAS.cSa w^+mC wg^Gal4
Constructs (9)
	P{GAL4-dpp.blk1} P{tubP-GAL4} P{UAS-arm.S10} P{UAS-CG13076.G} P{UAS-dally.J} P{UAS-dlp.HA} P{UAS-Notum.G} P{UAS-Notum.GT} P{UAS-sgg.S}
Genes (17)
	arm dally Dll dlp en Notum nub peb Scer\GAL4 sfl sgg T:Hsap\GALNT3 T:Hsap\MYC T:Ivir\HA1 tsh w wg
Insertions (6)
	P{EPg}Notum^EPg31259 P{GawB}459.2 P{GawB}ap^md544 P{GawB}sd^SG29.1 P{GawB}wg^Gal4 P{PZ}wg^rO727