Reference Report

Reference
Citation	Yan, C.M., Dobie, K.W., Le, H.D., Konev, A.Y., Karpen, G.H. (2002). Efficient recovery of centric heterochromatin P-element insertions in Drosophila melanogaster. Genetics 161(1): 217--229. (Export to RIS)
FlyBase ID	FBrf0149015
Publication Type	Research paper
PubMed ID	12019236
PubMed Abstract	Approximately one-third of the human and Drosophila melanogaster genomes are heterochromatic, yet we know very little about the structure and function of this enigmatic component of eukaryotic genomes. To facilitate molecular and cytological analysis of heterochromatin we introduced a yellow(+) (y(+))-marked P element into centric heterochromatin by screening for variegated phenotypes, that is, mosaic gene inactivation. We recovered >110 P insertions with variegated yellow expression from approximately 3500 total mobilization events. FISH analysis of 71 of these insertions showed that 69 (97%) were in the centric heterochromatin, rather than telomeres or euchromatin. High-resolution banding analysis showed a wide but nonuniform distribution of insertions within centric heterochromatin; variegated insertions were predominantly recovered near regions of satellite DNA. We successfully used inverse PCR to clone and sequence the flanking DNA for approximately 63% of the insertions. BLAST analysis of the flanks demonstrated that either most of the variegated insertions could not be placed on the genomic scaffold, and thus may be inserted within novel DNA sequence, or that the flanking DNA hit multiple sites on the scaffold, due to insertions within different transposons. Taken together these data suggest that screening for yellow variegation is a very efficient method for recovering centric insertions and that a large-scale screen for variegated yellow P insertions will provide important tools for detailed analysis of centric heterochromatin structure and function.
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Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (2)
	Ab(?;f)A887 In(2LR)O
Alleles (2)
	tsh⁰⁴³¹⁹ y^+mDint2
Balancers (1)
	CyO-P{SUPor-P}RR1
Constructs (1)
	P{SUPor-P}
Genes (4)
	idr Su(Ste) tsh y
Insertions (74)
	P{PZ}tsh⁰⁴³¹⁹ P{SUPor-P}A338 P{SUPor-P}A887 P{SUPor-P}B079 P{SUPor-P}B160 P{SUPor-P}B168 P{SUPor-P}B283 P{SUPor-P}B296 P{SUPor-P}B319 P{SUPor-P}B330 P{SUPor-P}B415 P{SUPor-P}B486 P{SUPor-P}B554 P{SUPor-P}B720 P{SUPor-P}B783.2 P{SUPor-P}B840.1 P{SUPor-P}B841 P{SUPor-P}B848 P{SUPor-P}B879.1 P{SUPor-P}B898 P{SUPor-P}B947 P{SUPor-P}B969 P{SUPor-P}C002 P{SUPor-P}C143 P{SUPor-P}C151 P{SUPor-P}C313.2 P{SUPor-P}C380 P{SUPor-P}C423 P{SUPor-P}C657 P{SUPor-P}C671 P{SUPor-P}C882 P{SUPor-P}C900 P{SUPor-P}C907 P{SUPor-P}C978.2 P{SUPor-P}D058 P{SUPor-P}D285 P{SUPor-P}D425 P{SUPor-P}D459.2 P{SUPor-P}D523 P{SUPor-P}E097 P{SUPor-P}E760 P{SUPor-P}F259 P{SUPor-P}F351 P{SUPor-P}F443 P{SUPor-P}F702 P{SUPor-P}F806.2 P{SUPor-P}G188.2 P{SUPor-P}G462.2 P{SUPor-P}H091 P{SUPor-P}H127 P{SUPor-P}I019 P{SUPor-P}I202 P{SUPor-P}I335 P{SUPor-P}I933.1 P{SUPor-P}I997.2 P{SUPor-P}J024 P{SUPor-P}J028.2 P{SUPor-P}J209 P{SUPor-P}J322 P{SUPor-P}J357 P{SUPor-P}J390 P{SUPor-P}J448 P{SUPor-P}J545 P{SUPor-P}J564 P{SUPor-P}J632.2 P{SUPor-P}J685 P{SUPor-P}J690.1 P{SUPor-P}J718 P{SUPor-P}J730.1 P{SUPor-P}J985.2 P{SUPor-P}K013.1 P{SUPor-P}K092 P{SUPor-P}K169 P{SUPor-P}K250.2
Natural transposons (18)
	297 1360 1731 blood copia Doc F-element G-element gypsy HeT-A hobo I-element mdg1 micropia opus Rt1a Stalker TART-element