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Reference
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| Citation |
Schenkel, H., Hanke, S., de Lorenzo, C., Schmitt, R., Mechler, B.M. (2002). P elements inserted in the vicinity of or within the Drosophila snRNP SmD3 gene nested in the first intron of the Ornithine
decarboxylase antizyme gene affect only the expression of SmD3. Genetics 161(2): 763--772. |
| FlyBase ID |
FBrf0149027 |
| Type of publication |
Research paper |
| Offprint Available |
No |
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External Crossreferences
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| PubMed ID |
12072471 |
| PubMed Abstract |
The Drosophila gene for snRNP SmD3 (SmD3) is contained in reverse orientation within the first intron of the Ornithine Decarboxylase
Antizyme (AZ) gene. Previous studies show that two closely linked P elements cause the gutfeeling phenotype characterized
by embryonic lethality and aberrant neuronal and muscle cell differentiation. However, the exact nature of the gene(s) affected
in the gutfeeling phenotype remained unknown. This study shows that a series of P inserts located within the 5'-untranslated
region (5'-UTR) of SmD3 or its promoter affects only the expression of SmD3. Our analysis reveals that the gutfeeling phenotype
associated with P elements inserted in the 5'-UTR of SmD3 results from amorphic or strongly hypomorphic mutations. In contrast,
P inserts in the SmD3 promoter region reduce the expression of SmD3 without abolishing it and produce larval lethality with
overgrown imaginal discs, brain hemispheres, and hematopoietic organs. The lethality of these mutations could be rescued by
an SmD3+ transgene. Finally, inactivation of AZ was obtained by complementing with SmD3+ the deficiency Df(2R)guf(lex47) that
uncovers both SmD3 and AZ. Interestingly, AZ inactivation causes a new phenotype characterized by late larval lethality and
atrophy of the brain, imaginal discs, hematopoietic organs, and salivary glands.
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| Biosis |
2002.403640 |
| Zoological record |
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Associated Information
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| Comments |
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| Text of personal communication |
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| Associated files |
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Related Publications
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Also Published As
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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Published In
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| Abbreviation |
Genetics |
| Title |
Genetics |
| Authors |
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| Volume range |
1- |
| Year range |
1916- |
| Page range |
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| Publisher |
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| Place of publication |
Austin, TX, etc |
| Language of publication |
English |
| ISBN/ISSN |
0016-6731 |
| CODEN |
GENTAE |
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Data from Reference
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| Aberrations (2)
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| Alleles (20)
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| Constructs (1)
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| Genes (4)
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| Insertions (7)
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