Reference Report

Reference
Citation	Stefancsik, R. (2002.5.22). l(2)k05909. (Export to RIS)
FlyBase ID	FBrf0149625
Publication Type	Personal communication to FlyBase
PubMed ID
PubMed Abstract
Text of Personal Communication	From raymund.stefancsik@XXXXXXXXXXXXXXX Wed May 22 22:35:46 2002 To: flybase-help@XXXXXXXXXXXXXXX Subject: l(2)k05909 Hi, I have some unpublished results which may be of interest to FlyBase and the Drosophila research community. Our genetic analysis of l(2)k05909 suggests that it is an allele of synaptotagmin (syt). In the attachement of this e-mail I am sending a txt file describing our experimental results. Best regards, \-- Raymund Stefancsik, Ph.D. Cutaneous Biology Research Center Massachusetts General Hospital/Harvard Medical School Building 149, 13th Street Charlestown, MA 02129 USA Phone: 617-724-8290 Fax: 617-726-4453 E-mail: raymund.stefancsik@XXXXXXXXXXXXXXX Genetic analysis of two insertions, l(2)k05909 and EP(2)1039, located close to Alp23B Our laboratory is interested in TGF-beta signal transduction, so we tested two P element insertions in the proximity of Alp23B (Activin Like Protein at 23B, FBgn0031461) as potential alleles of this gene. The results are summarized below. A) l(2)k05909 is lethal in trans to syt^T77 or deficiencies uncovering syt (see Table 1). The only lethal mutation on the syt^T77 chromosome is in syt, because it can be rescued by a synaptotagmin cDNA transgene (syt^elav.PD.), when homozygous or in trans to syt^N6 (Littleton et al., 1994, Calcium dependence of neurotransmitter release and rate of spontaneous vesicle fusions are altered in Drosophila synaptotagmin mutants. Proc. Natl. Acad. Sci. USA 91(23): 10888--10892 ^FBrf0077252). From the lack of complementation between l(2)k05909 and syt^T77 we conclude that l(2)k05909 (FBgn0022151) is an allele of synaptotagmin (syt, FBgn0004242). B) The sequence recovered from the 3' end of P element EP(2)1039 (FBti0010740, GenBank \#AQ254821) maps very close to the upstream region of the Alp23B transcribed region. EP(2)1039 is homozygous viable (see Table 1) with no apparent abnormalities. It is not an allele of Cy (FBgn0000403), which also maps to this region. _____________________________________________________ TABLE 1: l(2)k05909 EP(2)1039 Cy¹ Df(2L)C144 \- \+ \- Df(2L)JS31 \- \+ \- Df(2L)N6 \- \+ \+ Df(2)JS17 \+ \+ \+ syt^T77 \- nd \+ Cy^67.3 \+ \+ \- EP(2)1039 \+ \+ \+ _____________________________________________________ TABLE LEGEND: \+: viable in trans (with no visible abnormalities) \-: lethal in trans nd: not determined FLY STOCKS USED: y¹ w^67c23; P{w^+mC=lacW}l(2)k05909^k05909/CyO (SOURCE: Bloomington Stock Center, BL-10586) Df(2L)C144, dpp^d-ho ed¹/In(2LR)Gla, wg^Gla-1 Bc¹ Egfr^E1 (SOURCE: Bloomington Stock Center, BL-90) Dp(2;1)JS13, z¹ w^11E4; Df(2L)JS31, dpp^d-ho/CyO CyO (SOURCE: Bloomington Stock Center, BL-1581) y¹ w^; Df(2L)N6, P{w^+mC=lacW}B8-2-30, syt^N6/CyO (SOURCE: Bloomington Stock Center, BL-3910) Df(2L)JS17, dpp^d-ho/CyO, P{ry^+t7.2=en1}wg^en11 (SOURCE: Bloomington Stock Center, BL-1567) y¹ w^; P{w^+mC=lacW}syt^T77/CyO (SOURCE: Bloomington Stock Center, BL-4377) EP(2)1039/CyO (balancer is a guess R.S.) (SOURCE: Szeged Stock Center) Cy^67.3 dpp^d*/In(2LR)Gla, wg^Gla-1 (SOURCE: Bloomington Stock Center, BL-3918) \- Raymund Stefancsik, Ph.D. Cutaneous Biology Research Center Massachusetts General Hospital/Harvard Medical School Building 149, 13th Street Charlestown, MA 02129 USA Phone: 617-724-8290 Fax: 617-726-4453 E-mail: raymund.stefancsik@XXXXXXXXXXXXXXX
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Language of Publication	English
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Data from Reference
Aberrations (4)
	Df(2L)C144 Df(2L)JS17 Df(2L)JS31 Df(2L)N6
Alleles (4)
	Cy¹ Cy^67.3 Syt1^k05909 Syt1^T77
Genes (3)
	Cy daw Syt1
Insertions (2)
	P{EP}daw^EP1039 P{lacW}Syt1^k05909