Reference Report

Reference
Citation	Soustelle, L., Besson, M., Rival, T., Birman, S. (2002). Terminal glial differentiation involves regulated expression of the excitatory amino acid transporters in the Drosophila embryonic CNS. Dev. Biol. 248(2): 294--306. (Export to RIS)
FlyBase ID	FBrf0151251
Publication Type	Research paper
PubMed ID	12167405
PubMed Abstract	The Drosophila excitatory amino acid transporters dEAAT1 and dEAAT2 are nervous-specific transmembrane proteins that mediate the high affinity uptake of L-glutamate or aspartate into cells. Here, we demonstrate by colocalization studies that both genes are expressed in discrete and partially overlapping subsets of differentiated glia and not in neurons in the embryonic central nervous system (CNS). We show that expression of these transporters is disrupted in mutant embryos deficient for the glial fate genes glial cells missing (gcm) and reversed polarity (repo). Conversely, ectopic expression of gcm in neuroblasts, which forces all nerve cells to adopt a glial fate, induces an ubiquitous expression of both EAAT genes in the nervous system. We also detected the dEAAT transcripts in the midline glia in late embryos and dEAAT2 in a few peripheral neurons in head sensory organs. Our results show that glia play a major role in excitatory amino acid transport in the Drosophila CNS and that regulated expression of the dEAAT genes contributes to generate the functional diversity of glial cells during embryonic development.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Alleles (7)
	Ecol\lacZ^AA142 Ecol\lacZ^Kr.BoE Ecol\lacZ^wg-en11 gcm^Scer\UAS.cHa gcm^ΔP1 repo⁰³⁷⁰² Scer\GAL4^sca-537.4
Constructs (2)
	P{Kr-lacZ.BS23} P{UAS-gcm.H}
Genes (11)
	Eaat1 Eaat2 Ecol\lacZ elav eve futsch gcm pros repo Scer\GAL4 tup
Insertions (3)
	P{en1}wg^en11 P{GawB}sca^537.4 P{lwB}AA142