Reference Report

Reference
Citation	Brumby, A.M., Zraly, C.B., Horsfield, J.A., Secombe, J., Saint, R., Dingwall, A.K., Richardson, H. (2002). Drosophila cyclin E interacts with components of the Brahma complex. EMBO J. 21(13): 3377--3389. (Export to RIS)
FlyBase ID	FBrf0151282
Publication Type	Research paper
PubMed ID	12093739
PubMed Abstract	Cyclin E-Cdk2 is essential for S phase entry. To identify genes interacting with cyclin E, we carried out a genetic screen using a hypomorphic mutation of Drosophila cyclin E (DmcycE(JP)), which gives rise to adults with a rough eye phenotype. Amongst the dominant suppressors of DmcycE(JP), we identified brahma (brm) and moira (mor), which encode conserved core components of the Drosophila Brm complex that is highly related to the SWI-SNF ATP-dependent chromatin remodeling complex. Mutations in genes encoding other Brm complex components, including snr1 (BAP45), osa and deficiencies that remove BAP60 and BAP111 can also suppress the DmcycE(JP) eye phenotype. We show that Brm complex mutants suppress the DmcycE(JP) phenotype by increasing S phases without affecting DmcycE protein levels and that DmcycE physically interacts with Brm and Snr1 in vivo. These data suggest that the Brm complex inhibits S phase entry by acting downstream of DmcycE protein accumulation. The Brm complex also physically interacts weakly with Drosophila retinoblastoma (Rbf1), but no genetic interactions were detected, suggesting that the Brm complex and Rbf1 act largely independently to mediate G(1) arrest.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	EMBO J.
Title	The EMBO Journal
Publication Year	1982-
ISBN/ISSN	0261-4189
Data from Reference
Aberrations (5)
	Df(1)C246 Df(1)lz-90b24 Df(1)M38-C5 Df(2L)cl-h3 Df(3L)ZN47
Alleles (64)
	ash1²² ash1^B1 ash2¹ ash2¹⁸ Asx¹ BacA\p35^GMR.PH brm² brm^25S14 brm^E6S8 brm^{K804R.T:Ivir\HA1} btl¹ btl⁰⁰²⁰⁸ CycE^JP CycE^Scer\UAS.cRa E(Pc)¹ Hsc70-4⁰³⁵⁵⁰ Hsc70-4^L3929 hth^5E04 kis¹ kis⁰⁷⁸¹² kto¹ lawc^EF520 lid¹⁰⁴²⁴ lid^k06801 mod(mdg4)⁰³⁸⁵² mod(mdg4)^L3101 mor¹ mor^35S1 osa⁰⁰⁰⁹⁰ osa^ksh-11 osa^s3263b osa^Scer\UAS.cCa Pc¹ Pc² Pc⁴ Pc⁶ Pc⁷ Pcl¹¹ ph-d⁵⁰³ Psc¹ Rbf¹¹ Rbf^120a Rbf^GMR.PD Rbf^hs.PD Scer\GAL4^69B Scer\GAL4^Act5C.PI Scer\GAL4^GMR.PF Scer\GAL4^αTub84B.PL Scer\GAL80^αTub84B.PL Scm^D1 Scr¹ skd² sls¹ Snr1⁰¹³¹⁹ Snr1^{cdel.3.Scer\UAS} Snr1^R3 Trl^13C Trl⁶² Trl^R67 Trl^R85 trx¹ trx⁰⁰³⁴⁷ trx^E2 urd²
Constructs (11)
	P{Act5C-GAL4} P{brm^K804R.T:HA1} P{GAL4-ninaE.GMR} P{GMRP35} P{GMR-Rbf} P{hs-Rbf.D} P{tubP-GAL4} P{tubP-GAL80} P{UAS-CycE.R} P{UAS-osa} P{UAS-snr1.cdel.3}
Genes (35)
	ash1 ash2 Asx BacA\p35 Bap60 brm btl CycE dalao E(Pc) Hsc70-4 hth kis kto lawc lid mod(mdg4) mor osa Pc Pcl ph-d Psc Rbf Scer\GAL4 Scer\GAL80 Scm Scr skd sls Snr1 T:Ivir\HA1 Trl trx urd
Insertions (9)
	P{bluetail}Trl^13C P{CaSpeR}Rbf^120a P{GawB}69B P{lacW}mod(mdg4)^L3101 P{lacZ-ΔJP}CycE^JP P{PZ}Hsc70-4⁰³⁵⁵⁰ P{PZ}mod(mdg4)⁰³⁸⁵² P{PZ}osa⁰⁰⁰⁹⁰ P{PZ}Snr1⁰¹³¹⁹