Reference Report

Reference
Citation	Reich, A., Shilo, B.Z. (2002). Keren, a new ligand of the Drosophila epidermal growth factor receptor, undergoes two modes of cleavage. EMBO J. 21(16): 4287--4296. (Export to RIS)
FlyBase ID	FBrf0151286
Publication Type	Research paper
PubMed ID	12169631
PubMed Abstract	Spitz (Spi) is the most prominent ligand of the Drosophila EGF receptor (DER). It is produced as an inactive membrane precursor which is retained in the endoplasmic reticulum (ER). To allow cleavage, Star transports Spi to the Golgi, where it undergoes cleavage by Rhomboid (Rho). Since some DER phenotypes are not mimicked by any of its known activating ligands, we identified an additional ligand by database searches, and termed it Keren (Krn). Krn is a functional homolog of Spi since it can rescue the spi mutant phenotype in a Rho- and Star-dependent manner. In contrast to Spi, however, Krn also possesses a Rho/Star-independent ability to undergo low-level cleavage and activate DER, as evident both in cell culture and in flies. The difference in basal activity correlates with the cellular localization of the two ligands. While Spi is retained in the ER, the retention of Krn is only partial. Examining Spi/Krn chimeric and deletion constructs implicates the Spi cytoplasmic domain in inhibiting its basal activity. Low-level activity of Krn calls for tightly regulated expression of the Krn precursor.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	EMBO J.
Title	The EMBO Journal
Publication Year	1982-
ISBN/ISSN	0261-4189
Data from Reference
Alleles (24)
	Egfr^f5 Krn::spi^{SpiEC-KrnIC.Scer\UAS.T:Avic\GFP} Krn::spi^{SpiEC-KrnIC.Scer\UAS} Krn^MtnA.PR Krn^{s.Scer\UAS.cRa} Krn^Scer\UAS.cRa Krn^{Scer\UAS.T:Avic\GFP} Krn^{Δ16aa.Scer\UAS} Ras85D^e1B rho^{dsRNA.Scer\UAS} rho^MtnA.PR rho^PΔ38 S^IIN S^MtnA.PR Scer\GAL4^55B Scer\GAL4^69B Scer\GAL4^Bx-MS1096 Scer\GAL4^GMR.PF Sos^e2H spi³ spi^{Scer\UAS.T:Avic\GFP} spi^{Scer\UAS.ΔIC.T:Avic\GFP} spi^{Δ16aa.Scer\UAS} spi^{ΔIC.Scer\UAS.T:Zzzz\FLAG}
Constructs (12)
	P{GAL4-ninaE.GMR} P{UAS-Krn.R} P{UAS-Krn.s.R} P{UAS-Krn.T:GFP} P{UAS-Krn.Δ16aa} P{UAS-rho-dsRNA} P{UAS-spi.T:GFP} P{UAS-spi.Δ16aa} P{UAS-Spi.ΔIC.FLAG} P{UAS-spi.ΔIC.T:GFP} P{UAS-SpiEC::KrnIC} P{UAS-T:GFP.SpiEC::KrnIC}
Genes (11)
	Egfr Krn Krn::spi Ras85D rho S Scer\GAL4 Sos spi T:Avic\GFP T:Zzzz\FLAG
Insertions (3)
	P{GawB}69B P{GawB}Bx^MS1096 P{GawB}Eip63F-1^55B