Reference Report

Reference
Citation	Saller, E., Kelley, A., Bienz, M. (2002). The transcriptional repressor Brinker antagonizes Wingless signaling. Genes Dev. 16(14): 1828--1838. (Export to RIS)
FlyBase ID	FBrf0151325
Publication Type	Research paper
PubMed ID	12130542
PubMed Abstract	In the embryonic midgut of Drosophila, Wingless (Wg) signaling elicits threshold-specific transcriptional response, that is, low-signaling levels activate target genes, whereas high-signaling levels repress them. Wg-mediated repression of the HOX gene Ultrabithorax (Ubx) is conferred by a response sequence within the Ubx B midgut enhancer, called WRS-R. It further depends on the Teashirt (Tsh) repressor, which acts through the WRS-R without binding to it. Here, we show that Wg-mediated repression of Ubx B depends on Brinker, which binds to the WRS-R. Furthermore, Brinker blocks transcriptional activation by ubiquitous Wg signaling. Brinker binds to Tsh in vitro, recruits Tsh to the WRS-R, and we find mutual physical interactions between Brinker, Tsh, and the corepressor dCtBP. This suggests that the three proteins may form a ternary repressor complex at the WRS-R to quench the activity of the nearby-bound dTCF/Armadillo transcription complex. Finally, brinker and tsh produce similar mutant phenotypes in the ventral epidermis, and double mutants mimic overactive Wg signaling in this tissue. This suggests that Brinker may have a widespread function in antagonizing Wg signaling.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Alleles (12)
	brk^F124 brk^M68 brk^Scer\UAS.cLa Ecol\lacZ^Ubx.Bhz Ecol\lacZ^Ubx.BM2 Ecol\lacZ^Ubx.RPhz Scer\GAL4^arm.PS Scer\GAL4^how-24B tsh⁸ tsh^Scer\UAS.cGa wg^hs.PN wg^Scer\UAS.cLa
Constructs (8)
	P{Bhz} P{BM2} P{GAL4-arm.S} P{HS-wg} P{RPhz} P{UAS-brk.L} P{UAS-tsh.G} P{UAS-wg.L}
Genes (9)
	brk CtBP Ecol\lacZ ovo rho Scer\GAL4 tsh Ubx wg
Insertions (1)
	P{GawB}how^24B