Reference Report

Reference
Citation	Barolo, S., Stone, T., Bang, A.G., Posakony, J.W. (2002). Default repression and Notch signaling: hairless acts as an adaptor to recruit the corepressors Groucho and dCtBP to Suppressor of Hairless. Genes Dev. 16(15): 1964--1976. (Export to RIS)
FlyBase ID	FBrf0151327
Publication Type	Research paper
External Crossreferences
PubMed ID	12154126
PubMed Abstract	The DNA-binding transcription factor Suppressor of Hairless [Su(H)] functions as an activator during Notch (N) pathway signaling, but can act as a repressor in the absence of signaling. Hairless (H), a novel Drosophila protein, binds to Su(H) and has been proposed to antagonize N signaling by inhibiting DNA binding by Su(H). Here we show that, in vitro, H directly binds two corepressor proteins, Groucho (Gro) and dCtBP. Reduction of gro or dCtBP function enhances H mutant phenotypes and suppresses N phenotypes in the adult mechanosensory bristle. This activity of gro is surprising, because it is directed oppositely to its traditionally defined role as a neurogenic gene. We find that Su(H)-H complexes can bind to DNA with high efficiency in vitro. Furthermore, a H-VP16 fusion protein causes dominant-negative phenotypes in vivo, a result consistent with the proposal that H functions in transcriptional repression. Taken together, our findings indicate that "default repression" of N pathway target genes by an unusual adaptor/corepressor complex is essential for proper cell fate specification during Drosophila peripheral nervous system development.
BIOSIS ID	2002.442283
Zoological Record ID
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Editors
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Alleles (15)
	CtBP^87De-10 CtBP⁰³⁴⁶³ gro^E48 gro^E73 H²¹ H^E31 H^{N.Scer\UAS.T:Hsim\VP16} H^Scer\UAS.cGa H^{ΔC.Scer\UAS.T:Hsim\VP16} H^ΔC.Scer\UAS N^l1N-ts1 Scer\GAL4^sca-537.4 Scer\GAL4^Su(H).ASE5 Su(H)^RC-9Xm w^+mC
Constructs (6)
	P{Su(H)^RC-9Xm} P{Su(H)-GAL4.ASE5} P{UAS-H.G} P{UAS-H.N-VP16} P{UAS-H.ΔC} P{UAS-H.ΔC-VP16}
Genes (15)
	CtBP Dhyd\H dl en gro Gsc h H Kr N Scer\GAL4 Su(H) sv T:Hsim\VP16 w
Insertions (1)
	P{GawB}sca^537.4