Reference Report

Reference
Citation	Baines, R.A., Seugnet, L., Thompson, A., Salvaterra, P.M., Bate, M. (2002). Regulation of synaptic connectivity: levels of Fasciclin II influence synaptic growth in the Drosophila CNS. J. Neurosci. 22(15): 6587--6595. (Export to RIS)
FlyBase ID	FBrf0151382
Publication Type	Research paper
PubMed ID	12151538
PubMed Abstract	Much of our understanding of synaptogenesis comes from studies that deal with the development of the neuromuscular junction (NMJ). Although well studied, it is not clear how far the NMJ represents an adequate model for the formation of synapses within the CNS. Here we investigate the role of Fasciclin II (Fas II) in the development of synapses between identified motor neurons and cholinergic interneurons in the CNS of Drosophila. Fas II is a neural cell adhesion molecule homolog that is involved in both target selection and synaptic plasticity at the NMJ in Drosophila. In this study, we show that levels of Fas II are critical determinants of synapse formation and growth in the CNS. The initial establishment of synaptic contacts between these identified neurons is seemingly independent of Fas II. The subsequent proliferation of these synaptic connections that occurs postembryonically is, in contrast, significantly retarded by the absence of Fas II. Although the initial formation of synaptic connectivity between these neurons is seemingly independent of Fas II, we show that their formation is, nevertheless, significantly affected by manipulations that alter the relative balance of Fas II in the presynaptic and postsynaptic neurons. Increasing expression of Fas II in either the presynaptic or postsynaptic neurons, during embryogenesis, is sufficient to disrupt the normal level of synaptic connectivity that occurs between these neurons. This effect of Fas II is isoform specific and, moreover, phenocopies the disruption to synaptic connectivity observed previously after tetanus toxin light chain-dependent blockade of evoked synaptic vesicle release in these neurons.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Neurosci.
Title	Journal of Neuroscience
Publication Year	1981-
ISBN/ISSN	0270-6474 1529-2401
Data from Reference
Alleles (17)
	Avic\GFP^Scer\UAS.cUa Con^Scer\UAS.cCa Ctet\TeTxLC^{IMPTNT-V.Scer\UAS} Ctet\TeTxLC^TNT.Scer\UAS Ecol\lacZ^Fas2-A31 Fas1^Scer\UAS.cHa Fas2^e76 Fas2^e86 Fas2^e93 Fas2^EB112 Fas2^GPI.Scer\UAS Fas2^Scer\UAS.cLb Scer\GAL4^B19 Scer\GAL4^Cha.7.4 Scer\GAL4^eve.RRK Scer\GAL4^insc-Mz1407 Scer\GAL80^eve.RRK
Constructs (11)
	P{B19-GAL4} P{Cha-GAL4.7.4} P{eve.RRK-GAL80} P{eve-GAL4.RRK} P{UAS-Con.C} P{UAS-Fas1.H} P{UAS-Fas2.PEST+} P{UAS-Fas2^GPI.D} P{UAS-GFP.U} P{UAS-TeTxLC.(-)V} P{UAS-TeTxLC.tnt}
Genes (8)
	Avic\GFP Con Ctet\TeTxLC Ecol\lacZ Fas1 Fas2 Scer\GAL4 Scer\GAL80
Insertions (2)
	P{A92}A31 P{GawB}insc^Mz1407